H5147
单克隆抗-热休克蛋白 70 小鼠抗
clone BRM-22, ascites fluid
别名:
HSP70 Antibody - Monoclonal Anti-Heat Shock Protein 70 antibody produced in mouse, Hsp70 Antibody
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关于此项目
Conjugate:
unconjugated
Clone:
BRM-22, monoclonal
Application:
electron microscopy
immunohistochemistry (frozen sections)
indirect ELISA
microarray
western blot
immunohistochemistry (frozen sections)
indirect ELISA
microarray
western blot
Species reactivity:
bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster
Citations:
104
Technique(s):
electron microscopy: suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using bovine brain extract
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using bovine brain extract
Uniprot accession no.:
产品名称
单克隆抗-热休克蛋白 70 小鼠抗, clone BRM-22, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BRM-22, monoclonal
mol wt
antigen 70 kDa
species reactivity
bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster
technique(s)
electron microscopy: suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using bovine brain extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HSPA1A(3303), HSPA1B(3304)
rat ... Hspa1a(24472), Hspa1b(294254)
Application
小鼠源抗热休克蛋白70单克隆抗体可用于：
- 酶联免疫吸附测定（ELISA）
- 免疫印迹
- 斑点印迹
- 免疫细胞化学
已通过牛脑提取物免疫印迹法确定抗体滴度至少为1:5,000。
Biochem/physiol Actions
HSP70家族的成员在多肽的折叠、展开和易位以及寡聚蛋白复合物的组装和拆卸中起主要作用。另外，在免疫应答中，HSP70家族蛋白也有几种可能的作用。研究已表明，酒精性肝病与HSP70的胞浆内积累有关。研究发现HSP72在阿尔茨海默氏症患者的大脑中急剧增加，并且主要集中于神经炎斑块和神经原纤维缠结中。HSP70在热休克期间集中在细胞核中，并在热休克消除后返回细胞质。
通过免疫印迹，该抗体定位 HSP70 的组成型（HSP73）和诱导型（HSP72）形式。该抗体识别来自脑和其他组织的 HSP70。免疫荧光染色显示 HSP70 蛋白在热休克（42℃，1 小时）人成纤维细胞的细胞核内快速且可逆地积累。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
单克隆抗热休克蛋白70（小鼠IgG1同种型）分离自BRM-22杂交瘤，其通过融合小鼠骨髓瘤细胞和来自用缀合KLH的溴脱氧尿苷免疫的BALB/c小鼠的脾细胞产生。热休克蛋白家族A（Hsp70）成员1A（HSPA1A）由映射到人类染色体6p21.33上的基因编码。它在所有组织中广泛表达。所编码的蛋白具有N末端45 kDa的ATPase结构域和C末端25 kDa的底物结合域。
多种环境破坏（例如温度突然升高）会诱导细胞迅速合成一组多肽，这些多肽被称为热休克（应激）蛋白。真核细胞包含一个多基因家族，该家族可编码几个密切相关的70kD应激蛋白（HSP70家族），它们在细胞内的位置和调控方式有所不同。该家族包括四种蛋白：组成型（或同源）HSP73、应激诱导型HSP72和葡萄糖调节蛋白grp78（或BiP）和grp75。
Immunogen
从牛脑中分离的 HSP70
Other Notes
本品为采用细胞培养杂交瘤生产的纯化产品。
SAB4200714抗热休克蛋白 70 (HSP70) 抗体，小鼠单克隆
从杂交瘤细胞培养物中纯化的克隆BRM-22
SAB4200714抗热休克蛋白 70 (HSP70) 抗体，小鼠单克隆
从杂交瘤细胞培养物中纯化的克隆BRM-22
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Bacterial lipopolysaccharide augments febrile-range hyperthermia-induced heat shock protein 70 expression and extracellular release in human THP1 cells
Tulapurkar ME, et al.
Testing, 10(2), e0118010-e0118010 (2015)
Anne Marie Bakke-McKellep et al.
The British journal of nutrition, 97(4), 699-713 (2007-03-14)
Soyabean meal (SBM)-induced enteritis in the distal intestine of the teleost Atlantic salmon (Salmo salar L.) and other salmonids may be considered a model for diet-related mucosal disorders in other animals and man. The role of the intestinal microbiota in
Ling Wang et al.
PloS one, 9(10), e109465-e109465 (2014-10-21)
The soybean pod borer (Leguminivora glycinivorella Matsumura) successfully survives the winter because of its high expression of 70-kDa heat shock proteins (HSP70s) during its overwintering diapause. The amount of HSP70s is different under different environmental stresses. In this study, inducible
M P Mayer et al.
Cellular and molecular life sciences : CMLS, 62(6), 670-684 (2005-03-17)
Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments
Mohan E Tulapurkar et al.
PloS one, 10(2), e0118010-e0118010 (2015-02-07)
Sepsis, a devastating and often lethal complication of severe infection, is characterized by fever and dysregulated inflammation. While infections activate the inflammatory response in part through Toll-like receptors (TLRs), fever can partially activate the heat shock response with generation of
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