A3687
抗 兔 IgG(全分子)-碱性磷酸酶 山羊抗
affinity isolated antibody, buffered aqueous glycerol solution
别名:
Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody
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About This Item
推荐产品
生物来源
goat
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous glycerol solution
种属反应性
rabbit
技术
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
IgG是糖蛋白抗体,可调节几种免疫反应。针对靶蛋白的兔IgG通常在各种研究应用中用作初级抗体。因此,与可检测底物偶联的次级抗兔IgG是分析靶蛋白的有用工具。山羊抗兔IgG(全分子)-碱性磷酸酶抗体结合所有兔Igs。
免疫原
纯化兔 IgG 作为免疫原
应用
使用碱性磷酸酶缀合的山羊抗兔IgG作为二抗(用5%吐温/PBS按1:2000稀释),采用ELISA法分析小鼠视网膜组织中存在的低氧探针结合蛋白的水平。使用的底物是4-硝基苯基磷酸二钠盐六水合物(Sigma)。
山羊抗兔IgG(全分子)-碱性磷酸酶抗体可用于免疫印迹和ELISA。该抗体也可用于免疫组化(1:50,使用福尔马林固定、石蜡包埋切片)。
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用,使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液(Ph 9.6)中以1:5000稀释的二次稀释剂。
外形
在含有1mM MgCl 2,10mM甘氨酸,1%牛血清白蛋白,50%甘油和15mM叠氮化钠的0.05M Tris缓冲液(pH 8.0)中的溶液。
免责声明
除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Pro-inflammatory cytokines are important mediators of β-cell demise in type 1 diabetes, and similar mechanisms are increasingly implicated in type 2 diabetes, where a state of chronic inflammation may persist. It is likely that the actions of anti-inflammatory cytokines are
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The Lyme disease spirochete
G Cibelli et al.
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Rod photoreceptor loss in Rho-/- mice reduces retinal hypoxia and hypoxia-regulated gene expression.
Tanyth E de Gooyer et al.
Investigative ophthalmology & visual science, 47(12), 5553-5560 (2006-11-24)
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