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描述
Separated on Discovery® BIO PolyMA-WAX
技术
HPLC: suitable
试验参数
column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
detector: UV, 280 nm
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
适用性
application for HPLC
应用
life science and biopharma
分析说明
The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Trypsin Inhibitor
标准
产品编号
说明
价格
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