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Merck
CN

796-0657

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

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About This Item

描述

Separated on Discovery® BIO PolyMA-WAX

技术

HPLC: suitable

试验参数

column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
detector: UV, 280 nm
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

适用性

application for HPLC

应用

life science and biopharma

分析说明

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

法律信息

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

说明

    Trypsin Inhibitor

标准

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