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生物来源
equine skeletal muscle
产品线
BioUltra
方案
95-100% (SDS-PAGE)
表单
essentially salt-free, lyophilized powder
分子量
~17 kDa(lit.)
纯化方式
affinity chromatography
铁含量含量
~0.30%
技术
mass spectrometry (MS): suitable
溶解性
H2O: soluble 10 mg/mL
UniProt登记号
储存温度
−20°C
基因信息
horse ... MB(100054434)
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应用
肌红蛋白被用作质谱和X射线晶体学分析的分子量标记物和标准品。来自马骨骼肌的肌红蛋白被用于测试实验蛋白质混合物以验证串联质谱分析。
马骨骼肌中的肌红蛋白可用于诱发小鼠急性肾损伤(AKI)。它可用作自上而下质谱分析的标准物。
生化/生理作用
来自马骨骼肌的肌红蛋白是一种含有153个氨基酸残基的单链血红素蛋白。 它不含二硫键或游离-SH基团。肌红蛋白含有8个不同大小的右旋螺旋区域，由无序或随机的卷曲区域连接。
肌红蛋白对于近最大需氧量的骨骼肌O2 供应至关重要，并且通过将PO2维持在支持线粒体功能所需水平以上来防止缺氧。
肌红蛋白对于近最大需氧量的骨骼肌O2 供应至关重要，并且通过将PO2维持在支持线粒体功能所需水平以上来防止缺氧。
肌红蛋白对骨骼肌在接近最大氧气需求时的O2 的供应至关重要，并通过将PO2 维持在高于支持线粒体功能所需的水平来防止缺氧。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
S C Powell et al.
Journal of chromatography, 317, 87-92 (1984-12-28)
Urine and serum myoglobin have been separated on an anion-exchange column, packed by the slurry technique. Urine or serum was injected directly into the column and eluted isocratically with Tris buffer. Freshly prepared myoglobin from human muscle gives two peaks
Seok Jong Song et al.
International journal of molecular sciences, 21(22) (2020-11-19)
A recent study showed that early renal tubular injury is ameliorated in Nod-like receptor pyrin domain-containing protein 3 (NLRP3) KO mice with rhabdomyolysis-induced acute kidney injury (RIAKI). However, the precise mechanism has not been determined. Therefore, we investigated the role
Z K Shihabi
Journal of chromatography. B, Biomedical applications, 669(1), 53-58 (1995-07-07)
Capillary electrophoresis was used in this study to separate urinary myoglobin from hemoglobin based on its electrophoretic mobility. Urine was applied directly without any treatment. The separation was accomplished in less than 7 min. Myoglobin extracted from human muscle tissues
Protein Bioinformatics (2017)
J Zaia et al.
Rapid communications in mass spectrometry : RCM, 6(1), 32-36 (1992-01-01)
Myoglobins from horse heart muscle, horse skeletal muscle and sperm whale are widely used as calibration standards or test compounds for various mass spectrometric methodologies. In all such cases reported in the literature, a molecular weight value is used (16,950.5
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