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一般描述
25%的卵类粘蛋白由糖类组成。
卵类粘蛋白或胰蛋白酶抑制剂是大多数禽蛋白中富含的蛋白质。鸡蛋白约由186个氨基酸残基组成,且高度糖基化。三个串联的结构域均与胰蛋白酶抑制剂同源。其免疫原性很高,而且可能是大多数鸡蛋过敏的原因。
应用
胰蛋白酶抑制剂(来源于鸡蛋白)已用于:
- 生产变性、氧化和脱糖基化卵类粘蛋白,用于刺激患者来源细胞培养物以进行T细胞表位作图
- 处理核酸进行流式细胞分析
- Holtfreter溶液,用于分离涡虫细胞
单位定义
在25℃、pH 7.6条件下,反应体积为3.2 mL、光程为1 cm时,以BAEE为底物,一单位胰蛋白酶每分钟可产生0.001的ΔA253 。
分析说明
1 mg可抑制0.8-1.6 mg活性约为10,000单位BAEE/mg蛋白的胰蛋白酶。可抑制≤0.3 mg活性约为40单位BAEE/mg蛋白的胰凝乳蛋白酶。
其他说明
来自鸡蛋白的卵类粘蛋白本身不会抑制胰凝乳蛋白酶。根据Feeney, R. E.等人 J. Biol. Chem., 238, 1415 (1963)中的方法,胰凝乳蛋白酶抑制可检测卵抑制剂污染。
查看更多关于 胰蛋白酶抑制剂的信息。
警示用语:
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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