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Merck
CN

T9003

Sigma-Aldrich

胰蛋白酶抑制剂 来源于大豆

lyophilized powder

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别名:
SBTI
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

Glycine max (soybean)

质量水平

形式

lyophilized powder

分子量

20.1 kDa

溶解性

H2O: >10 mg/mL
phosphate buffer: >10 mg/mL (hazy above 10 mg/ml)
soluble

应用

diagnostic assay manufacturing

储存温度

2-8°C

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相关类别

应用

胰蛋白酶用于研究评估一种碱性蛋白酶在动物细胞培养中的潜在应用,该蛋白酶来自弧菌Vibrio sp. V26的非产毒红树林分离株。 胰蛋白酶也用于研究改善嗜酸性粘蛋白中真菌的检测。

生化/生理作用

该抑制剂能够在较小程度上抑制胰蛋白酶、胰凝乳蛋白酶和纤溶酶。它还能抑制蛋白酶血浆激肽释放酶和凝血因子X,机制与抑制胰蛋白酶类似。胰蛋白酶抑制剂不会抑制金属蛋白酶、基于组织的激肽释放酶、酸性蛋白酶或硫代蛋白酶。该抑制剂通过与蛋白酶活性位点按1:1化学计量形成复合物,然后在抑制剂上切割单个精氨酸-异亮氨酸键。抑制作用是可逆的且具有pH依赖性。

组分

大豆胰蛋白酶抑制剂是一种单体蛋白,在两个二硫键交联的单个多肽链中含有181个氨基酸残基。

单位定义

一个胰蛋白酶单位是指在25°C、pH 7.6条件下,以N-α-苯甲酰基-L-精氨酸乙酯(BAEE)为底物,每分钟产生0.001的 A253所需的酶量。
在25℃、pH 7.6条件下,反应体积为3.2 ml、光程为1 cm时,以BAEE为底物,一单位胰蛋白酶每分钟可产生0.001的ΔA253

制备说明

胰蛋白酶抑制剂能够以10 mg/mL溶于水和磷酸盐缓冲液。它能够以1 mg/mL溶于平衡盐溶液和无血清培养基中。浓度大于10 mg/mL的浓缩液可能浑浊,呈黄色至琥珀色。使用胰蛋白酶消化细胞后,重悬于胰蛋白酶抑制剂溶液中,使得用于解离的胰蛋白酶溶液浓度达到1 mg/mL。然后将细胞悬浮液以1000rpm离心,得到细胞沉淀。

在2-8℃下短期储存时,溶液可保持活性。溶液分装后可在-20℃冷冻并保持稳定。

分析说明

1 mg胰蛋白酶抑制剂可抑制1.0-3.0 mg活性为~10,000单位BAEE/mg蛋白的胰蛋白酶。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?   

    Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference:     J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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实验方案

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Chromatograms

application for HPLC

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