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OGS107

Sigma-Aldrich

PSF-CMV-PUC18 - CMV PUC18 MCS PLASMID

plasmid vector for molecular cloning

别名:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

表单

buffered aqueous solution

分子量

size 4310 bp

菌种筛选

ampicillin

复制起点

pUC (500 copies)

肽切割

no cleavage

启动子

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

报告基因

none

运输

ambient

储存温度

−20°C

一般描述

A versatile cloning plasmid for the expression of genes in mammalian cells. This plasmid contains the multiple cloning site (MCS) from pUC18 however it has been modified slightly to accommodate some restriction sites in our vector system. These changes are described in the full plasmid details PDF. The use of this MCS instead of the our normal vector MCS will limit the ability to use some of the inserts that we sell that immediately flank or are inserted within the standard MCS. This primarily includes N-terminal tags and signal peptides. This is because these inserts are flanked by restriction sites that are not compatible with the pUC18 MCS. Most other inserts should still be compatible with this plasmid.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.

应用

Multiple cloning site notes: This vector contains a different multiple cloning site (MCS) from the standard SnapFast vector. The MCS is derived from pUC18. The order of the restriction sites in this vector are almost the same as in pUC18 however the sequence is not. The pUC18 MCS has also been modified as follows: The XbaI site in the pUC18 MCS has been replaced with a SpeI site (these sites produce compatible cohesive ends when cleaved).

  • The SbfI and PstI sites have been replaced with an NsiI site (which produces compatible cohesive ends with SbfI and PstI when cleaved).
  • The BamHI site that is normally found in all SnapFast vectors (in the downstream fusion MCS) has been replaced with a BclI site because the pUC18 MCS contains this site. BamHI and BclI produce compatible cohesive ends when cleaved although BclI is methylated in this vector and will require growth in a Dam methylase negative bacterial strain (such as JM110 cells) before it can be used.
  • The ClaI to NheI sites have other functions such as adding peptide tags or IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site. This concept is explained in more detail on our website using the drop down box on the homepage and selecting: Fuse a coding sequence to the 3 prime end of a gene already in the plasmid.
  • The ClaI to NheI sites have other functions such as adding peptide tags or IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site. This concept is explained in more detail on our website using the drop down box on the homepage and selecting: Fuse a coding sequence to the 3 prime end of a gene already in the plasmid.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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价格

储存分类代码

12 - Non Combustible Liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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