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OGS2767

PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID

Name: PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

别名:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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UNSPSC代码:

12352200

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此商品	OGS3176	OGS3181	OGS2806
OGS2767 PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID	OGS3176 PSF-OXB20-COOH-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID	OGS3181 PSF-OXB20-COOH-HA - C-TERMINAL HA TAG BACTERIAL PLASMID	OGS2806 PSF-OXB20-NH2-10HIS-EKT - N-TERMINAL 10HIS TAG BACTERIAL PLASMID
peptide tag location C-terminal	peptide tag location C-terminal	peptide tag location C-terminal	peptide tag location N-terminal
promoter Promoter name: OXB20 Promoter activity: constitutive Promoter type: bacterial	promoter Promoter name: OXB20 Promoter activity: constitutive Promoter type: bacterial	promoter Promoter name: OXB20 Promoter activity: constitutive Promoter type: bacterial	promoter Promoter name: OXB20 Promoter activity: constitutive Promoter type: bacterial
bacteria selection kanamycin	bacteria selection kanamycin	bacteria selection kanamycin	bacteria selection kanamycin
peptide cleavage TEV	peptide cleavage no cleavage	peptide cleavage no cleavage	peptide cleavage EKT
origin of replication pUC (500 copies)	origin of replication pUC (500 copies)	origin of replication pUC (500 copies)	origin of replication pUC (500 copies)
mol wt size 3905 bp	mol wt size 3884 bp	mol wt size 3881 bp	mol wt size 4982 bp

一般描述

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

储存分类代码

12 - Non Combustible Liquids

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Not applicable

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The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.

Alexander C Cerny et al.

PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)

Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

In vivo characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity.

Geoffrey M Lynn et al.

Nature biotechnology, 33(11), 1201-1210 (2015-10-27)

The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer

Dickkopf-3 regulates prostate epithelial cell acinar morphogenesis and prostate cancer cell invasion by limiting TGF-β-dependent activation of matrix metalloproteases.

Diana Romero et al.

Carcinogenesis, 37(1), 18-29 (2015-10-28)

Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we

Notch3 interactome analysis identified WWP2 as a negative regulator of Notch3 signaling in ovarian cancer.

Jin-Gyoung Jung et al.

PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)

The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

商品

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

Sequencing Primers

Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.

DNA Component Explanation

Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.

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PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID

plasmid vector for molecular cloning

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