OGS412
PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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1 ML
¥603.26
10 ML
¥4,533.44
¥603.26
国内现货，预计发货时间2025年4月14日详情
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1 ML
¥603.26
10 ML
¥4,533.44
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
¥603.26
国内现货，预计发货时间2025年4月14日详情
推荐产品
表单
buffered aqueous solution
分子量
size 5475 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
启动子
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
报告基因
firefly luciferase
运输
ambient
储存温度
−20°C
1 of 4
此商品 | RTC000103 | 41639 | D2438 |
|---|---|---|---|
| application(s) cleaning products | application(s) pharmaceutical small molecule | application(s) - | application(s) - |
| format neat | format - | format - | format - |
| Quality Level 100 | Quality Level 300 | Quality Level 200 | Quality Level 400 |
| grade analytical standard | grade certified reference material, pharmaceutical secondary standard | grade for molecular biology | grade BioPerformance Certified |
| assay ≥99.95% (GC) | assay - | assay ≥99.5% (GC) | assay - |
一般描述
The expression of the Firefly luciferase (FLuc Photinus pyralis) reporter gene under the control of the RecA constitutive bacterial promoter. This plasmid can be used for monitoring reporter gene activity in bacterial cells or colonies using the luciferase substrate luciferin.
Promoter Expression Level: PSF-OXB20-FLUC - BACTERIAL LUCIFERASE plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
Promoter Expression Level: PSF-OXB20-FLUC - BACTERIAL LUCIFERASE plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
应用
Cloning in a gene: PSF-OXB20-FLUC - BACTERIAL LUCIFERASE plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.
Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.
BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
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产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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商品
SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..
Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.
Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.
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