推荐产品
生物来源
Escherichia coli
等级
for molecular biology
生长模式
adherent or suspension
形态学
rod shaped
技术
microbiological culture: suitable
细胞转化
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
运输
dry ice
储存温度
−70°C
相关类别
一般描述
BL21（DE3）化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因，可用于含有T7启动子的任何表达质粒。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
应用
该 大肠杆菌 BL21（DE3）菌株已用于：以pET19b作为表达载体，表达tau 2N4R同种型（aa1–441）和截短的tau（aa1–421）。BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。
特点和优势
- 转化效率> 1×107 CFU /µg
- 实惠的价格
- 可为日常蛋白表达工作提供卓越价值。
组分
- BL21（DE3）化学感受态细胞
- pUC19转化对照DNA
- 表达恢复培养基
相关产品
产品编号
说明
价格
WGK
WGK 3
法规信息
高风险级别生物产品--病毒，疫苗等
David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
Yasaman Damestani et al.
Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
Gints Kalnins et al.
Nature communications, 11(1), 388-388 (2020-01-22)
Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
相关内容
BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
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