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主要文件

安全信息

CMC0014

Sigma-Aldrich

BL21（DE3）化学感受态细胞

Escherichia coli, rod shaped

别名:

Competent E. coli cells

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1 MG
CN¥188.61
5 MG
CN¥932.18
10 MG
CN¥1,447.79
50 MG
CN¥6,302.72

CN¥188.61

国内现货，预计发货时间April 07, 2025详情

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1 MG
CN¥188.61
5 MG
CN¥932.18
10 MG
CN¥1,447.79
50 MG
CN¥6,302.72

About This Item

UNSPSC代码:
41106202
NACRES:
NA.85

CN¥188.61

国内现货，预计发货时间April 07, 2025详情

产品名称

BL21（DE3）化学感受态细胞, for protein expression

生物来源

Escherichia coli

等级

Molecular Biology
for molecular biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

运输

dry ice

储存温度

−70°C

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此商品
MBD0015MBD0020D8417
DAPI for nucleic acid staining

D9542

DAPI

DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

MBD0015

DAPI ready made solution

DAPI ready made solution with Antifade For Nuclear counterstain in Fluorescence Microscopy

MBD0020

DAPI ready made solution with Antifade

4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence

D8417

4′,6-Diamidino-2-phenylindole dihydrochloride

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

technique(s)

cell culture | mammalian: suitable

grade

for molecular biology

grade

-

grade

-

grade

-

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

solubility

H2O: 20 mg/mL, PBS: insoluble

solubility

water: soluble

solubility

-

solubility

H2O: 20 mg/mL, PBS: insoluble

form

powder

form

liquid

form

liquid

form

powder

一般描述

BL21（DE3）化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因，可用于含有T7启动子的任何表达质粒。

基因型

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

应用

该 大肠杆菌 BL21（DE3）菌株已用于：以pET19b作为表达载体，表达tau 2N4R同种型（aa1–441）和截短的tau（aa1–421）。[1]
  • BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。[2]
  • 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。[3][1]

    • 特点和优势

    • 转化效率> 1×107 CFU /µg
    • 实惠的价格
    • 可为日常蛋白表达工作提供卓越价值。

    组分

    • BL21（DE3）化学感受态细胞
    • pUC19转化对照DNA
    • 表达恢复培养基

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    H9950

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    CMC0015

    BL21（DE3）pLysE化学感受态细胞, for protein expression

    CMC0017

    过表达 ™ C41 (DE3) 化学感受态细胞, for the highest protein expression

    CMC0023

    CONTROLLER SIG10化学感受态细胞, for controlled protein expression

    CMC0024

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    CMR0001

    Recovery Medium for Expression, for use with competent cells

    储存分类代码

    10 - Combustible liquids

    法规信息

    高风险级别生物产品--病毒，疫苗等
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000346689
    SLCM7271
    SLCM4785
    SLCP0759
    SLCN4860

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    如果您需要特殊版本，可通过批号或批次号查找具体证书。

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    访问文档库

    David B Sauer et al.
    eLife, 9 (2020-09-02)
    Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
    Yasaman Damestani et al.
    Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
    The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
    A validated antibody panel for the characterization of tau post-translational modifications.
    Ebru E, et al.
    Mol. Neurodegener., 12(1), 87-87 (2017)
    Romana Koszagova et al.
    Microbial cell factories, 17(1), 139-139 (2018-09-05)
    Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
    Gints Kalnins et al.
    Nature communications, 11(1), 388-388 (2020-01-22)
    Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
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    BL21 Chemically Competent Cells Overview

    BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

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