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安全信息

SHC001

Sigma-Aldrich

MISSION®pLKO.1-puro空载体对照质粒 DNA

Contains no shRNA insert

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别名:
MISSION® 对照载体
MDL编号:
MFCD07785395
UNSPSC代码:
12352200
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

MISSION pLKO.1-puro 对照载体是一种慢病毒质粒载体。该载体不含 shRNA 插入，在使用 MISSION shRNA 文库克隆的实验中用作阴性对照。 这让人们可以检测转染对基因表达的影响，并解读 shRNA 克隆看到的敲低效应。氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。普尔科。1-puro 对照载体以 10μ 提供g 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl。

应用

MISSION® pLKO.1-puro 空载体对照质粒 DNA 已被用于合成和克隆 shRNA（靶向鼠分化簇 36 (CD36)），以生成慢病毒表达载体。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。

法律信息

使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Where is the hairpin inserted with Product SHC001, MISSION® pLKO.1-puro Control Vector?

    This control vector does not contain an shRNA insert.

  4. What is the function of this negative control with Product SHC001, MISSION® pLKO.1-puro Control Vector?

    It permits observation of cellular effects resulting from the transfection process. Cells transfected with the empty control provide a useful reference point for determining specific knockdown.

  5. Does Product SHC001, MISSION® pLKO.1-puro Control Vector, convey puromycin resistance to transfected cells?

    Yes, it has the puromycin resistance gene.

  6. Is Product SHC001, MISSION® pLKO.1-puro Control Vector, available as a glycerol stock?

    The control vectors are available in plasmid DNA or the lentiviral transduction format.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Cardiovascular Diabetology, 14 (2015)
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Timothy Andrew Couttas et al.
Metabolites, 10(6) (2020-06-12)
The number, position, and configuration of double bonds in lipids affect membrane fluidity and the recruitment of signaling proteins. Studies on mammalian sphingolipids have focused on those with a saturated sphinganine or mono-unsaturated sphingosine long chain base. Using high-resolution liquid
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shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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