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一般描述
MISSION pLKO.1-puro非靶标shRNA对照质粒DNA是一种慢病毒质粒载体。载体含有不靶向任何物种任何已知基因的shRNA（小发卡状RNA）插入片段，适合在使用MISSION shRNA文库克隆的实验中用作阴性对照。可用于检测转染的小发卡RNA对基因表达的影响，并解读shRNA克隆的敲低效应。氨苄青霉素和嘌呤霉素抗生素抗性基因分别用于细菌和哺乳动物细胞的选择性筛选。此外，利用表达载体与相容包装质粒共转染包装细胞(HEK293T)，可生产自灭活复制缺陷病毒颗粒。非靶标shRNA对照质粒DNA产品为溶于Tris-EDTA(TE)缓冲液的500 ng/μl溶液，含有10 μg质粒DNA。
应用
MISSION® pLKO.1-puro非靶标shRNA对照质粒DNA已用作以下应用的转导对照：
- 转导肿瘤细胞用于多色成像
- 转导成人低钙诱导角质形成(HaCaT)细胞
- 转导小鼠胚胎成纤维细胞，研究IP6K1（肌醇六磷酸激酶）的生物学功能
- 研究星形胶质细胞分化中的Zac1（调节细胞凋亡和细胞周期阻滞的锌指蛋白）表达
法律信息
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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说明
价格
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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