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此商品 | 57092 | 57128 | 57094 |
|---|---|---|---|
| matrix active group carbon phase | matrix active group carbon phase | matrix active group carbon phase | matrix active group carbon phase |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| particle size 120-400 mesh | particle size 120-400 mesh | particle size 120-400 mesh | particle size 120-400 mesh |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
| material polypropylene tube | material polypropylene tube | material polypropylene tube | material polypropylene tube |
| volume 3 mL | volume 6 mL | volume 12 mL | volume 6 mL |
一般描述
The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones.[1][2] This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
应用
To see more application data, protocols, vector maps visit sigma.com/shrna.
法律信息
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
推荐
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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