SHC016V
MISSION®
Targets no known genes from any species
别名:
MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
产品名称
MISSION®, Targets no known genes from any species
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
shipped in
dry ice
storage temp.
−70°C
Quality Level
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General description
当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
Analysis Note
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
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This study aimed at investigating the function and significance of CD110 expression in pancreatic cancer. We performed immunohistochemical staining for CD110 expression in tumor samples from 86 patients with pancreatic cancer. We evaluated clinical outcomes and other clinicopathological factors to
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