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Merck
CN

SHC001V

Sigma-Aldrich

MISSION®pLKO.1-puro空载体对照转导颗粒

Contains no shRNA insert

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别名:
MISSION®, MISSION® 对照转导颗粒
UNSPSC代码:
12352200
NACRES:
NA.51

质量水平

100
200

产品线

MISSION®

浓度

≥1x106 VP/ml (via p24 assay)

技术

capture ELISA: 106 TU/mL using p24

运输

dry ice

储存温度

−70°C

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一般描述

当使用MISSION® TRC shRNA克隆进行实验时,选择适当对照品是您的实验设计的关键要素,以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

应用

想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

法律信息

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

新产品

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Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor
Nature, 099655-099655 (2017)
Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4.
Miura N
Oncotarget (2016)
Deepak Parashar et al.
Cancer research, 80(24), 5554-5568 (2020-10-23)
Peritoneal spread is the primary mechanism of metastasis of ovarian cancer, and survival of ovarian cancer cells in the peritoneal cavity as nonadherent spheroids and their adherence to the mesothelium of distant organs lead to cancer progression, metastasis, and mortality.
Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (-)-Gossypol (AT-101).
Mani J
BMC Cancer, 15, 224-224 (2015)
Xiaobo Luo et al.
The Journal of clinical investigation, 130(4), 1635-1652 (2019-12-25)
The incidence of human papillomavirus-positive (HPV+) head and neck squamous cell carcinoma (HNSCC) has surpassed that of cervical cancer and is projected to increase rapidly until 2060. The coevolution of HPV with transforming epithelial cells leads to the shutdown of

商品

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

实验方案

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导,以实现稳定的长期沉默和表型变化的详细操作步骤。

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