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此商品 | FD2000S | FD40S | FD250S |
|---|---|---|---|
| biological source synthetic | biological source synthetic (organic) | biological source synthetic (organic) | biological source bacterial (Leuconostoc mesenteroides) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder or crystals | form powder | form powder | form powder |
| conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate |
| mol wt average mol wt 2,000,000 | mol wt average mol wt 2,000,000 | mol wt average mol wt 40,000 | mol wt average mol wt 250,000 |
| extent of labeling 0.001-0.020 mol FITC per mol glucose (substitution) | extent of labeling 0.003-0.020 mol FITC per mol glucose | extent of labeling 0.003-0.020 mol FITC per mol glucose | extent of labeling 0.003-0.020 mol FITC per mol glucose |
一般描述
MISSION ® 荧光素酶 shRNA 对照载体是一个 7,091 碱基对的慢病毒质粒载体，含有靶向来自 Photinus pyralis 荧光素酶的 shRNA 序列（GenBank 登记号：M15077)。荧光素酶 shRNA 对照载体在使用表达萤火虫荧光素酶的细胞系的实验中作为阳性敲除对照十分有用。其也可作为阴性对照载体。[1][2][3]
氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒，MISSION ® 慢病毒包装混合物（产品编号：SHP001）。荧光素酶 shRNA 对照载体以 10μg 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl.
氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒，MISSION ® 慢病毒包装混合物（产品编号：SHP001）。荧光素酶 shRNA 对照载体以 10μg 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl.
应用
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
法律信息
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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