推荐产品
一般描述
我们全面的shRNA产品系列包括超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。该文库可用于：基因家族集合：与特定功能类（如激酶和离子通道）相关的基因集合；基因靶标集合，针对靶标基因的shRNA克隆集合；以及单个shRNA克隆。我们的shRNA和慢病毒制造平台利用专门实验室，配备最先进的机器人技术、液体处理和实验室信息管理系统（LIMS），为您的RNAi研究需求提供卓越的质量和服务。
MISSION® TRC shRNA纯化质粒DNA形式省去了从每个构建体制备DNA的复杂操作。DNA可以直接用于瞬时或稳定转染。 另外，这些质粒可用于在包装细胞系中与包装质粒一起产生慢病毒颗粒。为靶标组中的每个构建体提供1μg DNA。
应用
MISSION® shRNA已被用于：
- ZO-1（胞质紧密粘连蛋白1）敲低
- 在等基因MCF10模型中敲低LIF（白血病抑制因子）表达
- 在n MIN6胰腺β细胞中敲低红细胞衍生核因子2样蛋白1
其他说明
想要查看实验方案和应用数据，请访问 sigma.com/shrna。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Deficiency of long isoforms of Nfe2l1 sensitizes MIN6 pancreatic beta cells to arsenite-induced cytotoxicity
Cui Q, et al.
Toxicology and Applied Pharmacology, 329(2), 67-74 (2017)
Stage-specific roles of FGF2 signaling in human neural development
Grabiec M, et al.
Stem Cell Research, 17(2), 330-341 (2016)
Epigenetic up-regulation of leukemia inhibitory factor (LIF) gene during the progression to breast cancer
Shin JE, et al.
Molecules and Cells, 31(2), 181-189 (2011)
Harry G Sherman et al.
Biochimica et biophysica acta. Bioenergetics, 1860(8), 628-639 (2019-06-24)
Trans-plasma membrane electron transfer (tMPET) is a process by which reducing equivalents, either electrons or reductants like ascorbic acid, are exported to the extracellular environment by the cell. TPMET is involved in a number of physiological process and has been
Alexandra M Young et al.
Molecular biology of the cell, 31(15), 1551-1560 (2020-05-28)
Nuclear membrane rupture during interphase occurs in a variety of cell contexts, both healthy and pathological. Membrane ruptures can be rapidly repaired, but these mechanisms are still unclear. Here we show barrier-to-autointegration factor (BAF), a nuclear envelope protein that shapes
商品
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
相关内容
Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.
寻找单独基因的shRNA超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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