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Application
MISSION® shRNA已被用于：
- ZO-1（胞质紧密粘连蛋白1）敲低
- 在等基因MCF10模型中敲低LIF（白血病抑制因子）表达
- 在n MIN6胰腺β细胞中敲低红细胞衍生核因子2样蛋白1
General description
我们全面的shRNA产品系列包括超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。该文库可用于：基因家族集合：与特定功能类（如激酶和离子通道）相关的基因集合；基因靶标集合，针对靶标基因的shRNA克隆集合；以及单个shRNA克隆。我们的shRNA和慢病毒制造平台利用专门实验室，配备最先进的机器人技术、液体处理和实验室信息管理系统（LIMS），为您的RNAi研究需求提供卓越的质量和服务。
MISSION® TRC shRNA纯化质粒DNA形式省去了从每个构建体制备DNA的复杂操作。DNA可以直接用于瞬时或稳定转染。 另外，这些质粒可用于在包装细胞系中与包装质粒一起产生慢病毒颗粒。为靶标组中的每个构建体提供1μg DNA。
Legal Information
使用本产品需遵守一个或多个 许可协议。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
想要查看实验方案和应用数据，请访问 sigma.com/shrna。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Epigenetic up-regulation of leukemia inhibitory factor (LIF) gene during the progression to breast cancer
Shin JE, et al.
Molecules and Cells, 31(2), 181-189 (2011)
Deficiency of long isoforms of Nfe2l1 sensitizes MIN6 pancreatic beta cells to arsenite-induced cytotoxicity
Cui Q, et al.
Toxicology and Applied Pharmacology, 329(2), 67-74 (2017)
Stage-specific roles of FGF2 signaling in human neural development
Grabiec M, et al.
Stem Cell Research, 17(2), 330-341 (2016)
Eva Ramos-Fernández et al.
Scientific reports, 11(1), 7395-7395 (2021-04-03)
Wnt signaling plays a key role in neurodevelopment and neuronal maturation. Specifically, Wnt5a stimulates postsynaptic assemblies, increases glutamatergic neurotransmission and, through calcium signaling, generates nitric oxide (NO). Trying to unveil the molecular pathway triggering these postsynaptic effects, we found that
Zhonglun Liu et al.
Oncology letters, 17(4), 3783-3789 (2019-03-19)
Resveratrol is one of the most promising phytoalexins for use as an anti-cancer agent, which is present in the skin of red grapes and berries. Resveratrol has been demonstrated to modulate a number of signalling pathways that are involved in
商品
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
相关内容
Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.
寻找单独基因的shRNA超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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