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OGS1120

PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID

Name: PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

别名:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC代码:

12352200

NACRES:

NA.85

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此商品	GERPN2209	GERPN2134	GERPN2106
GERPN2109 ECL^™ Blotting Reagents	GERPN2209 ECL^™ Western Blotting Detection Reagents	GERPN2134 ECL^™ Western Blotting Reagents	GERPN2106 ECL^™ Western Blotting Reagents
packaging pkg of 1 kit	packaging pkg of 1 kit	packaging pkg of 1 kit	packaging pkg of 1 kit
manufacturer/tradename Cytiva RPN2109	manufacturer/tradename Cytiva RPN2209	manufacturer/tradename Cytiva RPN2134	manufacturer/tradename Cytiva RPN2106
color Detection reagent 1: Colorless Detection reagent 2: Colorless	color Detection reagent 1: Colorless Detection reagent 2: Colorless	color Detection reagent 1: Colorless Detection reagent 2: Colorless	color Detection reagent 1: Colorless Detection reagent 2: Colorless
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

一般描述

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

储存分类代码

12 - Non Combustible Liquids

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Not applicable

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Notch3 interactome analysis identified WWP2 as a negative regulator of Notch3 signaling in ovarian cancer.

Jin-Gyoung Jung et al.

PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)

The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

In vivo characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity.

Geoffrey M Lynn et al.

Nature biotechnology, 33(11), 1201-1210 (2015-10-27)

The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer

Dickkopf-3 regulates prostate epithelial cell acinar morphogenesis and prostate cancer cell invasion by limiting TGF-β-dependent activation of matrix metalloproteases.

Diana Romero et al.

Carcinogenesis, 37(1), 18-29 (2015-10-28)

Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we

The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.

Alexander C Cerny et al.

PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)

Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

商品

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

Sequencing Primers

Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.

DNA Component Explanation

Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.

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PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

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