OGS553
PSF-OXB1 - WEAK STRENGTH BACTERIAL PROMOTER PLASMID
plasmid vector for molecular cloning
别名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
登录查看公司和协议定价
选择尺寸
1 ML
¥483.36
¥483.36
国内现货，预计发货时间2025年4月27日详情
所有图片(2)
选择尺寸
变更视图
1 ML
¥483.36
About This Item
UNSPSC代码:
12352200
NACRES:
NA.85
¥483.36
国内现货，预计发货时间2025年4月27日详情
推荐产品
重组
expressed in E. coli
表单
buffered aqueous solution
分子量
size 3859 bp
菌种筛选
kanamycin
复制起点
pUC (500 copies)
肽切割
no cleavage
启动子
Promoter name: OXB1
Promoter activity: constitutive
Promoter type: bacterial
报告基因
none
运输
ambient
储存温度
−20°C
1 of 4
此商品 | F8636 | F9378 | F2637 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line BioXtra | product line - | product line BioXtra |
| type Type 400-DL | type - | type Type 400-DL | type - |
organelle preparation, centrifugation gradients | organelle preparation, centrifugation gradients | - | - |
一般描述
This plasmid contains a weak bacterial promoter for expression in E. coli. It has been derived by modifying the AraBAD (arabinose operon) promoter to remove the AraC repressor site. When tested this promoter was found to be very weak in comparison to most other bacterial promoters including those in our product range. For this reason the promoter is called OXB1 in our collection of bacterial promoters that extend from OXB1 to OXB20 with OXB20 being the strongest. These promoters do not require induction for expression.
Promoter Expression Level: This plasmid contains a weak constitutive E. coli promoter that was derived from the Arabinose operon. It is part of our constitutive bacterial promoter range. This promoter (OXB1) shows the lowest level of expression in the range with OXB20 showing the highest level of expression. They require no inducing agent for expression.
Promoter Expression Level: This plasmid contains a weak constitutive E. coli promoter that was derived from the Arabinose operon. It is part of our constitutive bacterial promoter range. This promoter (OXB1) shows the lowest level of expression in the range with OXB20 showing the highest level of expression. They require no inducing agent for expression.
应用
Cloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
相关产品
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
商品
SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..
Versatile sequencing primers enable sequencing of inserts in plasmids at specific positions, aiding in molecular biology research.
Plasmid platform with interchangeable DNA components offers versatile research tools for genetic studies.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持
