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一般描述
质粒pBR322是构建用于大肠埃希菌的首批多用途克隆载体之一。该质粒衍生自ColE1型质粒pMB1，并具有与ColE1及其亲属相同的复制机制和对照。 质粒pBR322赋予对氨苄青霉素和四环素的抗性。质粒序列已发表。
质粒在氨苄西林耐药基因（Pst I、Pvu I和Sca I）、四环素耐药基因（BamH I、BspM I、EcoR V、Nhe I、Nru I、Sal I、Sph I和Xma III）和其他基因（Aat II、Ava I、Bal I、Bsm I、BspM II、Cla I、EcoR I、Hind III、Nde I、Pvu II、Ssp I、Sty I和Tth111 I）内具有独特的酶切位点。
质粒在氨苄西林耐药基因（Pst I、Pvu I和Sca I）、四环素耐药基因（BamH I、BspM I、EcoR V、Nhe I、Nru I、Sal I、Sph I和Xma III）和其他基因（Aat II、Ava I、Bal I、Bsm I、BspM II、Cla I、EcoR I、Hind III、Nde I、Pvu II、Ssp I、Sty I和Tth111 I）内具有独特的酶切位点。
特异性
唯一位点：在氨苄西林抗性基因内：Pst I，Pvu I，Sca I；在四环素抗性基因内：BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III；其他唯一位点：Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
应用
从大肠埃希菌中提取的pBR322质粒DNA RRI已被用作低分子量DNA，用于测试翡翠贻贝部分纯化组分的酶活性。它还被用于通过扫描电子显微镜研究考察其对碳酸钙颗粒形态的作用。
质粒pBR322是构建用于大肠埃希菌的首批多用途克隆载体之一。 该质粒和衍生物已用于许多目的，包括克隆、选择和表达重组分子，构建穿梭载体和用于核苷酸测序的载体、研究与基因表达有关的元素、作为质粒DNA标准品和模型系统，用于原核质粒复制的研究。
生化/生理作用
该质粒衍生自ColE1型质粒pMB1，并具有与ColE1及其亲属相同的复制机制和对照。
组分
DNA以粉末形式提供，由1 mM Tris-HCl (pH 7.5) + 1 mM NaCl和1 mM EDTA溶液冻干。
其他说明
登记号：J01749
登记号：J01749
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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