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表单
buffered aqueous solution
分子量
size 4361 bp
菌种筛选
ampicillin
复制起点
BR322 (15 copies)
肽切割
no cleavage
报告基因
none
运输
ambient
储存温度
−20°C
一般描述
该质粒含有 BR322（低拷贝数）复制起点，并为每个细菌细胞提供约 20 个质粒拷贝。它还包含用于选择转染哺乳动物细胞的MCS和Kan 抗性的哺乳动物CMV启动子上游。低拷贝数质粒对非常大的转基因或对细菌细胞有毒性的转基因是有用的。
启动子表达水平：
启动子表达水平：
应用
BR322 信息： BR322 是从大肠杆菌中分离得到的野生型质粒。其含有低拷贝来源，允许质粒以每个细胞约 10-100 个质粒拷贝的频率维持。通常每个细胞的这一数字接近 30-40个拷贝。最常见的克隆质粒含有该质粒中称为pUC的复制起点的衍生物。pUC 的起源是通过去除 Rep 阻遏蛋白形成的。阻遏蛋白通常在大肠杆菌中调节质粒拷贝数，也在复制起始点本身进行单点突变。点突变是复制起点之间最重要的差异。该质粒可作为通用低拷贝克隆载体。
序列
欲查看本品的序列信息，请访问 产品页面。
分析说明
欲查看本品的分析证书，请访问 www.oxgene.com
相关产品
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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