usage
kit sufficient for 100 histochemical assays
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
E. coli GUS 基因广泛用于分析转化植株中的基因表达。植物具有较低的内在 GUS 活性， 大肠杆菌 基因在植物细胞中相当稳定。作为标签，GUS 在融合蛋白的 N 端保持活性。
试剂盒适用于组织和细胞的生化活性测定、免疫学测定和组织化学染色。
试剂盒适用于组织和细胞的生化活性测定、免疫学测定和组织化学染色。
Features and Benefits
- 表达大肠杆菌 GUS 酶植物组织的组织化学染色
- 非常适合植物表达研究，因为植物中 GUS 活性非常低，酶稳定性高
- GUS 不干扰植物细胞功能或活力
General description
使用试剂盒分析 β 葡萄糖醛酸酶（E.coli GUS 基因）在转化植株中的表达。
Other Notes
本试剂盒所用底物为 X-GlcA（5-溴-4-氯-3-吲哚基 β- D -葡糖苷酸），导致转染细胞和组织中出现不溶性靛蓝沉淀。
Li-Ling Lin et al.
International journal of molecular sciences, 14(7), 14270-14286 (2013-07-11)
Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs
Kuo-Hsiang Chuang et al.
Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)
Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer
C-M Cheng et al.
Cancer gene therapy, 15(6), 393-401 (2008-03-29)
Increasing the specificity of chemotherapy may improve the efficacy of cancer treatment. Toward this aim, we developed a strain of bacteria to express enzymes for selective prodrug activation and non-invasive imaging in tumors. beta-glucuronidase and the luxCDABE gene cluster were
Ken Naito et al.
Nature, 461(7267), 1130-1134 (2009-10-23)
High-copy-number transposable elements comprise the majority of eukaryotic genomes where they are major contributors to gene and genome evolution. However, it remains unclear how a host genome can survive a rapid burst of hundreds or thousands of insertions because such
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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