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用途
kit sufficient for 100 histochemical assays
质量水平
技术
microbe id | staining: suitable
运输
dry ice
储存温度
−20°C
1 of 4
此商品 | GALS | CS0760 | LUC1 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) flow cytometry: suitable, protein staining: suitable | technique(s) - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
| usage kit sufficient for 100 histochemical assays | usage kit sufficient for 100 tests (using a 3.5 cm dish) | usage kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette) | usage kit sufficient for 100 assays |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
一般描述
使用试剂盒分析 β 葡萄糖醛酸酶（E.coli GUS 基因）在转化植株中的表达。
应用
E. coli GUS 基因广泛用于分析转化植株中的基因表达。植物具有较低的内在 GUS 活性， 大肠杆菌 基因在植物细胞中相当稳定。作为标签，GUS 在融合蛋白的 N 端保持活性。
试剂盒适用于组织和细胞的生化活性测定、免疫学测定和组织化学染色。
试剂盒适用于组织和细胞的生化活性测定、免疫学测定和组织化学染色。
特点和优势
- 表达大肠杆菌 GUS 酶植物组织的组织化学染色
- 非常适合植物表达研究，因为植物中 GUS 活性非常低，酶稳定性高
- GUS 不干扰植物细胞功能或活力
其他说明
本试剂盒所用底物为 X-GlcA（5-溴-4-氯-3-吲哚基 β- D -葡糖苷酸），导致转染细胞和组织中出现不溶性靛蓝沉淀。
仅试剂盒组分
产品编号
说明
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
R A Jefferson et al.
The EMBO journal, 6(13), 3901-3907 (1987-12-20)
We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We
Ken Naito et al.
Nature, 461(7267), 1130-1134 (2009-10-23)
High-copy-number transposable elements comprise the majority of eukaryotic genomes where they are major contributors to gene and genome evolution. However, it remains unclear how a host genome can survive a rapid burst of hundreds or thousands of insertions because such
Kuo-Hsiang Chuang et al.
Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)
Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer
C-M Cheng et al.
Cancer gene therapy, 15(6), 393-401 (2008-03-29)
Increasing the specificity of chemotherapy may improve the efficacy of cancer treatment. Toward this aim, we developed a strain of bacteria to express enzymes for selective prodrug activation and non-invasive imaging in tumors. beta-glucuronidase and the luxCDABE gene cluster were
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