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用途
kit sufficient for 100 histochemical assays
质量水平
技术
microbe id | staining: suitable
运输
dry ice
储存温度
−20°C
一般描述
使用试剂盒分析 β 葡萄糖醛酸酶（E.coli GUS 基因）在转化植株中的表达。
应用
E. coli GUS 基因广泛用于分析转化植株中的基因表达。植物具有较低的内在 GUS 活性， 大肠杆菌 基因在植物细胞中相当稳定。作为标签，GUS 在融合蛋白的 N 端保持活性。
试剂盒适用于组织和细胞的生化活性测定、免疫学测定和组织化学染色。
试剂盒适用于组织和细胞的生化活性测定、免疫学测定和组织化学染色。
特点和优势
- 表达大肠杆菌 GUS 酶植物组织的组织化学染色
- 非常适合植物表达研究，因为植物中 GUS 活性非常低，酶稳定性高
- GUS 不干扰植物细胞功能或活力
其他说明
本试剂盒所用底物为 X-GlcA（5-溴-4-氯-3-吲哚基 β- D -葡糖苷酸），导致转染细胞和组织中出现不溶性靛蓝沉淀。
仅试剂盒组分
产品编号
说明
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
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R A Jefferson
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The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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