usage
kit sufficient for 100 tests (using a 3.5 cm dish)
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
β-半乳糖苷酶(β-gal)催化X-gal向半乳糖和5-溴-4-氯-3-羟基吲哚的转化。5-溴-4-氯-3-羟基吲哚在铁氰化钾或亚铁氰化钾的存在下二聚化形成的蓝色沉淀可用于间接测定β-Gal活性。这种比色检测法还能使用其他β-gal底物，比如O-硝基苯-β-D-吡喃半乳糖苷(ONPG)。β-gal是一种经济简便的标准报告系统。半乳糖苷酶报告基因染色试剂盒提供了一种测定β-半乳糖苷酶基因LacZ表达质粒转染细胞百分比的方法。也可用于显微镜观察报告基因在组织切片中的特异性表达。
Other Notes
本试剂盒所用底物为 X-Gal（5-溴-4-氯-3-吲哚基 β- D -吡喃半乳糖苷），导致转染细胞和组织的靛蓝染色。本测试提供了一种方法，用于测定表达 Lac Z 的转染细胞百分比或显微观察组织切片中报告基因的表达。
Application
β-半乳糖苷酶报告基因染色试剂盒已用于动物细胞或组织的组化染色，包括：
- 矢状脑切片组织酶学
- 胰腺或结肠切片的β-半乳糖苷酶报告基因检测
- 小鼠E10.5 -11.5胚胎和胚胎切片
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
supp_hazards
存储类别
3 - Flammable liquids
flash_point_f
132.8 °F
flash_point_c
56 °C
D Long et al.
Journal of virology, 68(9), 5890-5898 (1994-09-01)
Human immunodeficiency virus type 1 (HIV-1) infects some cell types which lack CD4, demonstrating that one or more alternative viral receptors exist. One such receptor is galactosylceramide (GalCer), a glycosphingolipid distributed widely in the nervous system and in colonic epithelial
Maria Boichuck et al.
Aging, 11(9), 2889-2897 (2019-05-16)
Here, we reported for the first time an increased expression of c-Met protein in primary cultures of human dermal and pulmonary fibroblasts of late passages. This suggests that c-Met could serve as an early marker of cellular senescence (CS). The
An improved method with high sensitivity and low background in detecting low beta-galactosidase expression in mouse embryos
Shen X, et al.
PLoS ONE, 12(5), e0176915-e0176915 (2017)
GPR120 (FFAR4) is preferentially expressed in pancreatic delta cells and regulates somatostatin secretion from murine islets of Langerhans
Stone VM, et al.
Diabetologia, 57(6), 1182-1191 (2014)
beta-Galactosidase assay
Smale ST
Cold Spring Harbor Protocols, 2010, pdb-prot5423 (2010)
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