General description
萤火虫荧光素酶是基因表达中最常用的报告基因之一。这是一种极度灵敏、响应快速且易于使用的报告基因。荧光素酶催化的化学发光反应是测量基因表达最敏感的分析工具之一。与传统方法相比，荧光素酶作用物含有辅酶 A，可增加和维持发光。这可避免向自动光度计进样底物，并允许通过摄影胶片或闪烁计数法进行分析。裂解液含有多粘菌素 B，用于进一步增强信号并消除溶菌酶处理和细胞冻融。细胞裂解液兼容β-半乳糖苷酶测定。由荧光素酶报告基因编码的酶在ATP、氧和Mg2+环境下催化D-萤光素的氧化。定量荧光产物以测定其活性。
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Susan L Kozak et al.
The Journal of biological chemistry, 281(39), 29105-29119 (2006-08-05)
Deoxycytidine deaminases APOBEC3G (A3G) and APOBEC3F (A3F) (members of the apolipoprotein B mRNA-editing catalytic polypeptide 3 family) have RNA-binding motifs, invade assembling human immunodeficiency virus (HIV-1), and hypermutate reverse transcripts. Antagonistically, HIV-1 viral infectivity factor degrades these enzymes. A3G is
Rachel S Edgar et al.
Nature, 485(7399), 459-464 (2012-05-25)
Cellular life emerged ∼3.7 billion years ago. With scant exception, terrestrial organisms have evolved under predictable daily cycles owing to the Earth's rotation. The advantage conferred on organisms that anticipate such environmental cycles has driven the evolution of endogenous circadian rhythms
Gerben van Ooijen et al.
Current biology : CB, 21(10), 869-875 (2011-05-03)
Circadian clocks were, until recently, seen as a consequence of rhythmic transcription of clock components, directed by transcriptional/translational feedback loops (TTFLs). Oscillations of protein modification were then discovered in cyanobacteria. Canonical posttranslational signaling processes have known importance for clocks across
Giulia Donvito et al.
Neuropharmacology, 148, 320-331 (2018-03-24)
Cigarette smokers with brain damage involving the insular cortex display cessation of tobacco smoking, suggesting that this region may contribute to nicotine addiction. In the present study, we speculated that molecules in the insular cortex that are sensitive to experimental
Kai Qian et al.
Biotechnology letters, 37(11), 2229-2235 (2015-07-15)
To improve the bioactivity and increase the N-terminal homogeneity of a glucagon-like peptide-1 (GLP-1) analogue expressed in Pichia pastoris. The GLP-1 analogue. GGH, consisting of two tandem mutant GLP-1 (GLP-1[A2G]) fused with the N-terminus of human serum albumin (HSA), was
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Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
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