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一般描述
萤火虫荧光素酶是基因表达中最常用的报告基因之一。这是一种极度灵敏、响应快速且易于使用的报告基因。荧光素酶催化的化学发光反应是测量基因表达最敏感的分析工具之一。与传统方法相比，荧光素酶作用物含有辅酶 A，可增加和维持发光。这可避免向自动光度计进样底物，并允许通过摄影胶片或闪烁计数法进行分析。裂解液含有多粘菌素 B，用于进一步增强信号并消除溶菌酶处理和细胞冻融。细胞裂解液兼容β-半乳糖苷酶测定。由荧光素酶报告基因编码的酶在ATP、氧和Mg2+环境下催化D-萤光素的氧化。定量荧光产物以测定其活性。
警示用语：
Danger
危险声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Paola Monti et al.
Scientific reports, 10(1), 18427-18427 (2020-10-30)
Chronic lymphocytic leukaemia (CLL) is characterised by a heterogeneous clinical course. Such heterogeneity is associated with a number of markers, including TP53 gene inactivation. While TP53 gene alterations determine resistance to chemotherapy, it is not clear whether they can influence
Jaya Mishra et al.
Journal of the American Society of Nephrology : JASN, 14(10), 2534-2543 (2003-09-30)
Acute renal failure (ARF) secondary to ischemic injury remains a common and potentially devastating problem. A transcriptome-wide interrogation strategy was used to identify renal genes that are induced very early after renal ischemia, whose protein products might serve as novel
Kai Qian et al.
Biotechnology letters, 37(11), 2229-2235 (2015-07-15)
To improve the bioactivity and increase the N-terminal homogeneity of a glucagon-like peptide-1 (GLP-1) analogue expressed in Pichia pastoris. The GLP-1 analogue. GGH, consisting of two tandem mutant GLP-1 (GLP-1[A2G]) fused with the N-terminus of human serum albumin (HSA), was
Stephen T Smale
Cold Spring Harbor protocols, 2010(5), pdb-pdb (2010-05-05)
When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes
Rachel S Edgar et al.
Nature, 485(7399), 459-464 (2012-05-25)
Cellular life emerged ∼3.7 billion years ago. With scant exception, terrestrial organisms have evolved under predictable daily cycles owing to the Earth's rotation. The advantage conferred on organisms that anticipate such environmental cycles has driven the evolution of endogenous circadian rhythms
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Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.
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