应用
萤火虫荧光素酶在分子和细胞生物学中广泛使用，特别是用于 ATP 的有效检测和定量，并作为遗传功能的报告基因。
生化/生理作用
萤火虫荧光素酶是一种 62 kDa 的蛋白质，催化光的产生。这种酶需要 ATP、分子氧和杂环化合物萤火虫荧光素，在两步过程中产生光。光产生反应由荧光素活化（其羧基的腺苷酸化）启动，并在分子氧存在下进行，产生黄绿色光光子。
单位定义
一个荧光素酶单位在 20-25℃ 下将在10 秒内产生一个相对光单位 (RLU)，使用 GloMax 20/20 光度计在Tris-甘氨酸缓冲液中含有40pmol ATP和15nmol荧光素的100μl测定混合物中测定，溶液PH值为7.6。
单位定义换算系数：每个传统照明单位大约有 9000 个相对照明单位 (RLU)，在PPO / POPOP混合物中使用相当于0.02μ Ci 14 C 的峰高。
单位定义换算系数：每个传统照明单位大约有 9000 个相对照明单位 (RLU)，在PPO / POPOP混合物中使用相当于0.02μ Ci 14 C 的峰高。
外形
以含有 HEPES (pH 7.5)、NaCl、MgCl 2 、EDTA、DTT 的冻干粉和碳水化合物稳定剂形式提供。
本品是由 大肠杆菌表达的 luc 基因产生的来自 Photinus pyralis（美国萤火虫）的重组荧光素酶 (62 kDa) 。
制备说明
为了获得最大溶解度，重要的是在高盐浓度下复溶酶，如 1 MTris 缓冲液与 pH 7-8 的任何反离子。制备的酶浓度每毫升可高达 5 mg 蛋白。切勿涡旋，避免搅拌。
复溶后，酶溶液可在 4-8°C 下保存长达 2 天，或在-20°C 下以工作等份冷冻保存至少一个月。不推荐反复冻融。
复溶后，酶溶液可在 4-8°C 下保存长达 2 天，或在-20°C 下以工作等份冷冻保存至少一个月。不推荐反复冻融。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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