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B6650

Sigma-Aldrich

X-GlcA

reagent for selection of recombinant bacterial clones

别名:

5-溴-4-氯-3-吲哚基 β-D-葡糖醛酸 环己铵盐, X-GlcA, X-葡糖苷酸 CHA 盐

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100 μL
CN¥5,074.24

CN¥5,074.24

国内现货，预计发货时间April 14, 2025详情

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100 μL
CN¥5,074.24

About This Item

经验公式（希尔记法）:
C14H13BrClNO7 · C6H13N
CAS号:
114162-64-0
分子量:
521.79
MDL编号:
MFCD00058543
UNSPSC代码:
12352200
PubChem化学物质编号:
24891926
NACRES:
NA.85

CN¥5,074.24

国内现货，预计发货时间April 14, 2025详情

等级

Molecular Biology

质量水平

200

无菌性

non-sterile

方案

≥98% (TLC)

表单

powder

技术

nucleic acid detection: suitable

溶解性

DMF: soluble

适用性

suitable for β-galactosidase test

储存温度

−20°C

SMILES字符串

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

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此商品
B9285D5412I7125
5-溴-2′-脱氧尿苷 ≥99% (HPLC)

B5002

5-溴-2′-脱氧尿苷

5-溴-2′-脱氧尿苷 BioUltra, ≥99%

B9285

5-溴-2′-脱氧尿苷

2′-脱氧尿苷 ≥98.5%

D5412

2′-脱氧尿苷

5-碘-2′-脱氧尿苷 ≥99% (HPLC)

I7125

5-碘-2′-脱氧尿苷

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

assay

≥99% (HPLC)

assay

≥99%

assay

≥98.5%

assay

≥99% (HPLC)

form

powder

form

powder

form

powder

form

powder or crystals

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

solubility

NH4OH: 0.1 M at 20 °C, clear, colorless

solubility

water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

1 N NH4OH: 50 mg/mL, clear to very slightly hazy, colorless to light yellow

一般描述

X-GlcA 是 β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的 DNA 插入片段。 X-GlcA 设计用于具有 lac+ 表型的重组细菌菌落的蓝白色选择中替代 X-Gal。

应用

β-葡萄糖醛酸酶 (GUS) 基因检测的显色底物。
适用于选择具有 lac+ 表型的重组细菌菌落。
X-GlcA 已用于根切片的组化染色，用于光学显微镜观察。[1]

原理

当 X-GlcA 被 β-半乳糖苷酶水解时，所得产物将形成蓝色沉淀。在 X-GlcA 存在下生长的 Lac+ 菌落会变成深蓝色，从而易于区分 lac+ 和 lac- 菌落。
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    BCCK0770
    BCBR2021V
    BCBM9813V
    BCBW4472
    BCBW1488

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    Differential colonization of tomato roots by nonpathogenic and pathogenic Fusarium oxysporum strains may influence Fusarium wilt control
    Bao J R and Lazarovits G
    Phytopathology, 91(5), 449-456 (2001)
    Stéphane Compant et al.
    Applied and environmental microbiology, 71(4), 1685-1693 (2005-04-07)
    Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA
    Li Liu et al.
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 21(9), 2014-2019 (2007-03-14)
    Gene therapy shows promise for treating prostate cancer and has been evaluated in several clinical trials. A major challenge that remains is to establish a method for verifying transgene activity in situ. The lacZ gene encoding beta-galactosidase historically has been
    Quoc Phong Le et al.
    Foodborne pathogens and disease, 12(8), 719-725 (2015-06-26)
    Contamination of food with multiantibiotic-resistant bacteria, particularly extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, is considered a potential source for the wide dissemination of ESBL-producing bacteria in communities. However, little is known about the extent of contamination of food with ESBL-producing bacteria in
    Janine Beutlich et al.
    International journal of food microbiology, 209, 13-19 (2014-12-03)
    Foods of animal origin brought illegally from third party countries into the European Community pose a risk for the introduction of diseases. This can lead to animal disease outbreaks with significant economic and social costs and subsequent severe trade restrictions.
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