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Merck
CN

B6650

X-GlcA

reagent for selection of recombinant bacterial clones

别名:

5-溴-4-氯-3-吲哚基 β-D-葡糖醛酸 环己铵盐, X-GlcA, X-葡糖苷酸 CHA 盐

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关于此项目

经验公式（希尔记法）:
C14H13BrClNO7 · C6H13N
化学文摘社编号:
114162-64-0
分子量:
521.79
NACRES:
NA.85
PubChem Substance ID:
24891926
UNSPSC Code:
12352200
MDL number:
MFCD00058543

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产品名称

X-GlcA, reagent for selection of recombinant bacterial clones

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI key

JXCKZXHCJOVIAV-CYRSAHDMSA-N

grade

Molecular Biology

sterility

non-sterile

assay

≥98% (TLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

Quality Level

200

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相关类别

Biochem/physiol Actions

当 X-GlcA 被 β-半乳糖苷酶水解时，所得产物将形成蓝色沉淀。在 X-GlcA 存在下生长的 Lac+ 菌落会变成深蓝色，从而易于区分 lac+ 和 lac- 菌落。

General description

X-GlcA 是 β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的 DNA 插入片段。 X-GlcA 设计用于具有 lac+ 表型的重组细菌菌落的蓝白色选择中替代 X-Gal。

Application

β-葡萄糖醛酸酶 (GUS) 基因检测的显色底物。
适用于选择具有 lac+ 表型的重组细菌菌落。
X-GlcA 已用于根切片的组化染色，用于光学显微镜观察。

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN2572
BCCK0770
BCBR2021V
BCBM9813V
BCBW4472

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Stéphane Compant et al.
Applied and environmental microbiology, 71(4), 1685-1693 (2005-04-07)
Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA
Differential colonization of tomato roots by nonpathogenic and pathogenic Fusarium oxysporum strains may influence Fusarium wilt control
Bao J R and Lazarovits G
Phytopathology, 91(5), 449-456 (2001)
Li Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 21(9), 2014-2019 (2007-03-14)
Gene therapy shows promise for treating prostate cancer and has been evaluated in several clinical trials. A major challenge that remains is to establish a method for verifying transgene activity in situ. The lacZ gene encoding beta-galactosidase historically has been
S K Kritas et al.
Journal of animal science, 93(1), 405-413 (2015-01-09)
This field study assessed the efficacy of a probiotic based on viable spores of Bacillus subtilis C-3102 (Calsporin; Calpis Co. Ltd., Japan) on the health status and productivity of sows and their litters through 2 full, sequential reproductive cycles from
Quoc Phong Le et al.
Foodborne pathogens and disease, 12(8), 719-725 (2015-06-26)
Contamination of food with multiantibiotic-resistant bacteria, particularly extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, is considered a potential source for the wide dissemination of ESBL-producing bacteria in communities. However, little is known about the extent of contamination of food with ESBL-producing bacteria in
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