用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
neurological disorders; gastrointestinal diseases; hematological disorder
储存温度
−20°C
基因信息
human ... GBA(2629) , GBA2(57704) , GBA3(57733)
mouse ... GBA(14466) , GBA2(230101)
rat ... GBA(684536) , GBA2(298399) , GBA3(289687)
一般描述
β-葡糖苷酶可通过作用于末端非还原的b(1→4)-连接的D-葡萄糖残基来水解碳水化合物，同时释放D-葡萄糖。生物体需要β-葡糖苷酶来消化纤维素。溶菌酶，一种存在于泪液中的β-葡萄糖苷酶，可作用于革兰氏阴性细菌的肽聚糖细胞壁中存在的β(1→4)葡萄糖键，并有助于预防眼睛中的细菌感染。β-葡萄糖苷酶活性的缺失与戈谢病′和帕金森病′有关。
应用
β-葡萄糖苷酶活性测定试剂盒已用于测定样品的β-葡糖苷酶活性。
特点和优势
兼容高通量的操作系统。
适用性
β-葡萄糖苷酶活性检测试剂盒提供了一种对生物样品中的β-葡萄糖苷酶活性进行测定的简单直接的方案，可用于β-葡萄糖苷酶生成的性质分析，以及筛选β-葡萄糖苷酶的调控因子。
原理
在该检测中，β-葡糖苷酶活性通过β-葡糖苷酶水解 对硝基苯基-β-D-吡喃葡萄糖苷的反应来确定，该反应可形成比色(405 nm)产物，其与所存在的β-葡糖苷酶活性成比例。一个单位的β-葡糖苷酶表示在pH 7.0条件下，每分钟催化1.0 μmole底物水解所需的酶量。
储存分类代码
12 - Non Combustible Liquids
Spruce budworm (lepidoptera: tortricidae) oral secretions II: Chemistry.
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