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APOAF

Sigma-Aldrich

Annexin V-FITC 凋亡检测试剂盒

别名:

Annexin V-FITC, Apoptosis probe FITC

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.84

用途

(20 tests)

质量水平

300

包装

pkg of 1 kit

技术

flow cytometry: suitable

应用

cell analysis
detection

检测方法

fluorometric

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞，并使用流式细胞仪进行定量。AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯（FITC）偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。该试剂盒包含碘化丙啶(PI)，用于标记细胞膜完全破坏的坏死细胞内的DNA。这种组合可以区分早期凋亡细胞（annexin V阳性, PI阴性），坏死细胞（annexin V阳性, PI阳性），以及活细胞（annexin V阴性, PI阴性）。

应用

Annexin V-FITC 凋亡检测试剂盒适于以下应用：
  • 在治疗前列腺癌的过程中对LNCaP前列腺癌细胞染色以测定 G. lucidum提取物活性。
  • 在研究DBP-maf（维生素D结合蛋白-巨噬细胞活化因子）基因对前列腺癌细胞活性的抑制中，用于对肿瘤细胞的标记。
  • 用于对flippase酶活性的间接测定。

特点和优势

相比基于DNA的检测法（比如TUNEL法），可检测到细胞凋亡的更早期进程。
  • 快速标记细胞。细胞染色仅需10分钟。
  • 细胞无需固定或预处理，节省检测时间并满足细胞用于进一步研究。
  • 试剂盒内含碘化丙啶二级染料，用于从活细胞和坏死细胞中区分出凋亡细胞。

其他说明

使用前使所有组分达到室温。

仅试剂盒组分

产品编号
说明
  • APOAFA

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000389724
0000378389
0000378388
0000159278
0000378389

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. When using the Annexin V-FITC Apoptosis Detection Kit, Product APOAF, can I use any buffer for resuspending my cells?

    The binding buffer included in the kit needs to be used for resuspending cells.  The buffer contains calcium chloride at a final (1X) concentration of 2.5 mM which is necessary for the binding of annexin V to phosphatidylserine.

  4. What wavelength do I use to detect Annexin V-FITC and Propidium Iodide when using Annexin V-FITC Apoptosis Detection Kit, Product APOAF?

    Annexin V FITC will have a maximum emission of 528 nm. This can be measured in the standard FITC Channel on a flow cytometer (FL1).  Propidium Iodide has a maximum emission of 620 nm.  This is measured on the short red channel on a flow cytometer (FL2 or FL3).

  5. Can I use Product APOAF, Annexin V-FITC Apoptosis Detection Kit, to differentiate cells that are dead due to necrosis or apoptosis?

    When using a kinetic study (various time points), you can show the progression of the cells from viable (annexin V FITC negative, PI negative), to annexin FITC positive, PI negative (membrane flip) to annexin V FITC positive, PI positive (dead).  If there are cells that are double positive when starting, it is not possible to guarantee that the cell death occurred due to apoptosis with this assay.

  6. When using Product APOAF, Annexin V-FITC Apoptosis Detection Kit, I have cells that are Annexin V FITC negative and PI positive.  What are these cells?

    It is not possible to have cells that are PI positive without the cells also being positive for Annexin V binding.  Cells are PI positive because the membrane has been compromised.  If this is the case, Annexin V can also enter the cell and bind to the PS on the internal cell membrane. The gating on the histogram for the FITC channel should be changed so that all cells that are PI positive are also Annexin V positive.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Product APOAF, Annexin V-FITC Apoptosis Detection Kit be used on fixed cells?

    No. Product APOAF, Annexin V-FITC Apoptosis Detection Kit must be performed on live cells in order to measure Apoptosis. The assay is based on the externization of phosphatidylserine from the inner cell membrane to the outer cell membrane.  If the membrane is preturbed due to fixation, non-speciifc staining of the inner cell membrane might occur

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Denise C Arruda et al.
The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
Complementarity-determining regions (CDRs) from monoclonal antibodies tested as synthetic peptides display anti-infective and antitumor activities, independent of the specificity of the native antibody. Previously, we have shown that the synthetic peptide C7H2, based on the heavy chain CDR 2 from
Monika Kallubai et al.
Journal of biomolecular structure & dynamics, 37(3), 623-640 (2018-01-30)
Our study focus on the biological importance of synthesized 5β-dihydrocortisol (Dhc) and 5β-dihydrocortisol acetate (DhcA) molecules, the cytotoxic study was performed on breast cancer cell line (MCF-7) normal human embryonic kidney cell line (HEK293), the IC50 values for MCF-7 cells
Ben-Zion Zaidman et al.
International journal of oncology, 31(4), 959-967 (2007-09-06)
Ganoderma lucidum (Curt.:Fr.) P. Karst, a medicinal fungus, has been widely used in Asian countries for centuries to prevent or treat a variety of diseases, including cancer. However, the mechanisms responsible for the effects of G. lucidum on cancer cells
Juliana Rizzo et al.
Eukaryotic cell, 13(6), 715-726 (2013-12-18)
Flippases are key regulators of membrane asymmetry and secretory mechanisms. Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans. On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative
Kalvin J Gregory et al.
PloS one, 5(10), e13428-e13428 (2010-10-27)
Vitamin D binding protein-macrophage activating factor (DBP-maf) is a potent inhibitor of tumor growth. Its activity, however, has been attributed to indirect mechanisms such as boosting the immune response by activating macrophages and inhibiting the blood vessel growth necessary for
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