APOBRDU
Flow Cytometry Kit for Apoptosis
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
usage
sufficient for 60 cell suspensions
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Application
The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody.Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA. APOBRDU has been used in flow cytometry assay in human non–small cell lung cancer. APOBRDU has been used in cell cycle and apoptosis assay in HeLa cells, and in tunel assay human neuroblastoma cell lines.
Biochem/physiol Actions
Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3′-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3′-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.
Features and Benefits
Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.
Packaging
The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20 °C and APO-PART2 at 2-4 °C
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
3 - Flammable liquids
flash_point_f
55.4 °F
flash_point_c
13 °C
Regulatory Information
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
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