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Safety Information

APOAC

Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Synonym(s):

Red fluorescent Annexin V apoptosis assay

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Select a Size

20 μG
CN¥3,096.05
50 μG
CN¥5,521.21

CN¥3,096.05

Estimated to ship onJuly 21, 2025Details

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20 μG
CN¥3,096.05
50 μG
CN¥5,521.21

About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

CN¥3,096.05

Estimated to ship onJuly 21, 2025Details

usage

sufficient for 200 tests

Quality Level

200

packaging

pkg of 1 kit

technique(s)

flow cytometry: suitable

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

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MISSION® esiRNA targeting human SLC1A3

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MISSION® esiRNA

MISSION® esiRNA targeting human SLC9A7 (2)

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MISSION® esiRNA

esiRNA cDNA target sequence

GACCAGGTGGTCCTGTCCTACGGGGGCCTGCGCGGGGCCGTGGCCTTTGCCCTGGTGGTGCTTCTGGATGGAGACAAGGTCAAGGAGAAGAACCTGTTCGTCAGCACCACCATCATCGTAGTGTTCTTCACCGTCATCTTCCAGGGCCTGACCATCAAGCCTCTGGTGCAGTGGCTGAAGGTGAAGAGGAGCGAGCACCGGGAACCTCGGCTCAACGAGAAGCTGCACGGGCGCGCTTTCGACCACATCCTCTCGGCCATCGAGGACATATCCGGACAGATCGGGCACAATTATCTCAGAGACAAGTGGTCCCACTTCGACAGGAAGTTCCTCAGCAGGGTCCTCATGAGACGGTCGGCCCAGAAGTCTCGAGACCGGATCCTGAATGTCTTCCACGAGCTG

esiRNA cDNA target sequence

CACCACTGGAACTGGACCTTCGTCATCAGCACCCTGCTCTTCTGCCTCATCGCCCGCGTGCTGGGGGTGCTGGGCCTGACCTGGTTCATCAACAAGTTCCGTATCGTGAAGCTGACCCCCAAGGACCAGTTCATCATCGCCTATGGGGGCCTGCGAGGGGCCATCGCCTTCTCTCTGGGCTACCTCCTGGACAAGAAGCACTTCCCCATGTGTGACCTGTTCCTCACTGCCATCATCACTGTCATCTTCTTCACCGTCTTTGTGCAGGGCATGACCATTCGGCCCCTGGTAGACCTGTTGGCTGTGAAGAAAAAGCAAGAGACGAAGCGCTCCATCAACGAAGAGATCCACACACAGTTCCTGGACCACCTTCTGACAGGCATCGAAGACATCTGTGGCCACTACGGTCACCACCACTG

esiRNA cDNA target sequence

TTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAGATGCTGGTCTTACCACTTATCATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGTAAGGCATCAGGGAAGATGGGAATGCGAGCTGTAGTCTATTATATGACTACCACCATCATTGCTGTGGTGATTGGCATAATCATTGTCATCATCATCCATCCTGGGAAGGGCACAAAGGAAAACATGCACAGAGAAGGCAAAATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTGATCAGGAACATGTTCCCTCCAAATCTGGTAGAAGCCTGCTTTAAACAGTTTAAAACCAACTATGAGAAGAGAAGCTTTAAAGTGCCCATCCAGGCCAACGAAACGCTTGTGGGTGCTGTGATAAACAATGTGTCTGAGGCCATGGAGACTCT

esiRNA cDNA target sequence

TGGCAAGATCAACAGCGTAGAGCAGAATGATATGCTACGGAAGGTAACATTCGATCCAGAAGTATTTTTCAACATTCTTCTGCCTCCAATTATTTTTCATGCTGGATACAGCTTAAAGAAGAGACACTTTTTCAGAAATCTTGGATCTATACTGGCCTATGCCTTCTTGGGGACTGCTGTTTCATGCTTCATTATTGGAAATCTCATGTATGGTGTGGTGAAGCTCATGAAGATTATGGGACAGCTCTCAGATAAATTTTACTACACAGATTGTCTCTTTTTTGGAGCAATCATCTCTGCCACTGACCCAGTGACTGTGCTGGCGATATTTAATGAATTGCATGCAGACGTGGATCTTTACGCACTTCTTTTTGGAGAGAGCGTCCTAAATGATGCTGTTGCCATTGTACTGTCCTCGTCTATTGTTGCCTACCAGCCAGCGGGACTGAACACTCACGCCTTTGA

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

Gene Information

human ... SLC9A3(6550), SLC9A3(6550)

Gene Information

human ... SLC9A1(6548), SLC9A1(6548)

Gene Information

human ... SLC1A3(6507), SLC1A3(6507)

Gene Information

human ... SLC9A7(84679), SLC9A7(84679)

Ensembl | human accession no.

ENSG00000066230

Ensembl | human accession no.

ENSG00000090020

Ensembl | human accession no.

ENSG00000079215

Ensembl | human accession no.

ENSG00000065923

NCBI accession no.

NM_004174

NCBI accession no.

NM_003047

NCBI accession no.

NM_004172

NCBI accession no.

NM_032591

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of Cytiva

Kit Components Only

Product No.
Description
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Kit Components Also Available Separately

Product No.
Description
SDS
  • C50416-Carboxyfluorescein diacetate 10 mg

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000378384
    0000378384
    0000253320
    0000253320
    0000121791

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    Mohamed Asik R et al.
    Materials science & engineering. C, Materials for biological applications, 103, 109840-109840 (2019-07-28)
    Development of novel approach for cancer therapy, sparing healthy normal cells overcoming the limitation of available therapies is of prime importance for cervical cancer treatment. Recently metal oxide based chemotherapeutics has emanated as a promising approach for cancer therapy. Hence
    Andre F Steinert et al.
    Arthritis research & therapy, 11(5), R148-R148 (2009-10-06)
    The present study compares bone morphogenetic protein (BMP)-4 and BMP-2 gene transfer as agents of chondrogenesis and hypertrophy in human primary mesenchymal stem cells (MSCs) maintained as pellet cultures. Adenoviral vectors carrying cDNA encoding human BMP-4 (Ad.BMP-4) were constructed by
    J Dachary-Prigent et al.
    Biochemistry, 34(36), 11625-11634 (1995-09-12)
    The development of procoagulant activity and microparticle formation during platelet activation is known to depend on an increase in cytosolic Ca2+ levels. We have studied the mechanisms leading to these events using FITC-labeled recombinant annexin V, a protein which binds
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    Articles

    Apoptosis Assays

    Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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