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Merck
CN

C5041

6-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

Synonym(s):

6-CFDA, 6-Carboxy-di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
Molecular Weight:
460.39
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
67043
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Product Name

6-Carboxyfluorescein diacetate, ≥95% (HPLC)

Quality Level

assay

≥95% (HPLC)

form

powder

color

white to yellow

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

QMOGCCYGOPYYNT-UHFFFAOYSA-N

Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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M Kawai et al.
Journal of applied microbiology, 86(3), 496-504 (1999-04-10)
Physiologically active bacteria in purified water used in the manufacturing process of pharmaceutical products were enumerated in situ. Bacteria with growth potential were enumerated using the micro-colony technique and direct viable counting (DVC), followed by 24 h of incubation in
Yeli Gong et al.
PloS one, 7(10), e47435-e47435 (2012-10-19)
The MOG35-55 peptide-induced experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice is a useful animal model to explore therapeutic approaches to T cell-mediated autoimmune diseases because the dominant T-cell epitope(s) have been defined. It is rational that antigen-specific immunosuppression can
Sung-Uk Lee et al.
PloS one, 14(3), e0214253-e0214253 (2019-03-27)
N-acetylglucosamine (GlcNAc) branching of Asn (N)-linked glycans inhibits pro-inflammatory T cell responses and models of autoimmune diseases such as Multiple Sclerosis (MS). Metabolism controls N-glycan branching in T cells by regulating de novo hexosamine pathway biosynthesis of UDP-GlcNAc, the donor



Global Trade Item Number

SKUGTIN
C5041-25MG04061833501702
C5041-100MG04061833501696