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用途
100 assays in 96 well plates
运输
wet ice
储存温度
−20°C
一般描述
组蛋白乙酰转移酶（HAT）在细胞功能中发挥关键作用，它参与基因转录、分化和增殖的调控。HAT活性比色测定试剂盒提供了一种便捷的非放射性系统，可快速、灵敏地检测哺乳动物样品中的HAT活性。该试剂盒包含活性核提取物（NE）阳性对照、HAT辅因子、乙酰辅酶A以及所有下游辅因子和底物。活性HAT催化肽底物的乙酰化，释放出游离形式的辅因子，而辅因子是产生NADH所必需的辅酶。通过与可溶性四唑鎓染料反应，可使用分光光度法轻松检测到NADH。这种检测可以是连续性的，因此适用于动力学研究。该试剂盒为完整测定提供了所有试剂和简单的实验方案。
特点和优势
- 灵敏可靠的测定
- 利用比色法
- 样品类型：细胞和组织裂解液
- 种属反应性：哺乳动物
- 适用于独立测试或高通量分析和动力学研究
- 简便的过程：将核提取物或蛋白质样品与试剂混合、孵育并读取吸光度
- 方便的96孔微孔板规格
- 持续测量HAT活动；适用于动力学研究
- 无HDAC干扰；可直接使用核粗提物
储存分类代码
12 - Non Combustible Liquids
法规信息
监管及禁止进口产品
历史批次信息供参考:
Shigeki Shimba
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2119-2123 (2014-01-21)
Several epidemiological studies have suggested that the perturbation of circadian rhythm has adverse metabolic consequences (e.g., dyslipidemia) in humans. At the molecular level, circadian rhythms are encoded by an autoregulatory loop composed of a set of transcription activators (BMAL1/CLOCK) that
Atsuhito Nakao
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2153-2157 (2014-01-21)
IgE-mediated immediate-type allergic reactions in the skin show a diurnal rhythm, although the regulatory mechanisms are poorly understood. In mammals, the circadian rhythms are driven by the circadian clock system consisting of the light-entrained central clock located in the suprachiasmatic
Lisa N Chesner et al.
DNA repair, 97, 103026-103026 (2020-12-15)
While mammalian mitochondria are known to possess a robust base excision repair system, direct evidence for the existence of additional mitochondrial DNA repair pathways is elusive. Herein a PCR-based assay was employed to demonstrate that plasmids containing DNA-protein crosslinks are
Koji Maemura
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2124-2129 (2014-01-21)
Cardiovascular function exhibits circadian oscillation. Furthermore, the onset time and severity of cardiovascular diseases exhibit diurnal variation. The internal circadian clock whose center exists in the suprachiasmatic nucleus regulates this circadian variation. Many studies have reported that night shift workers
Michiko Konno
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2171-2178 (2014-01-21)
Mood disorders show a common feature of distorted cycling of biological systems, manifesting, for example, as diurnal mood variation in depression, phasic time course in bipolar disorder, and seasonal mood swing in seasonal affective disorder. Accordingly, circadian dysfunction has been
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