应用
组蛋白脱乙酰检测试剂盒提供了用于基于两步酶促反应的检测HDAC活性的简单方法。该酶促测定的底物是具有乙酰化赖氨酸残基和结合的荧光基团的取代肽。反应的第一步是通过含HDAC的样品（HeLa细胞提取物、纯化酶等）使乙酰化的赖氨酸侧链脱乙酰化。第二步是通过显影液裂解脱乙酰基的底物并释放游离的高荧光基团。测得的荧光与样品的脱乙酰活性成正比。
生化/生理作用
组蛋白脱乙酰基酶（HDAC）是从组蛋白中去除乙酰基的酶。HDAC是基因表达和基因组功能的重要调节剂。它们与多种人类疾病有关，如几种癌症、神经系统疾病和衰老等。因此，HDAC是治疗干预的相关关键目标。此外，已证明组蛋白脱乙酰基酶抑制剂可作为抗肿瘤剂。
特点和优势
该试剂盒包括快速简便地测量细胞或核提取物中或纯化的酶制剂中HDAC活性所需的所有试剂。此外，该试剂盒还提供HeLa细胞裂解物作为HDAC活性的来源，用于抑制剂筛选或作为阳性对照、HDAC抑制剂（曲古抑菌素A）和实现活性定量的标准品。
分析说明
组蛋白脱乙酰基酶测定试剂盒已经在HeLa、HEK 293T、NIH 3T3和U 973细胞提取物中进行了测试。
仅试剂盒组分
产品编号
说明
- Assay Buffer 20 mL
- Developer Solution 1.5 mL
- HDAC Inhibitor (Trichostatin A), 1 mM 50 μL
- HDAC Substrate, 20 mM 50 μL
- HeLa Cell Lysate 500 μL
- Standard (non-acetylated substrate), 4 mM 50 μL
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
高风险级别生物产品--病毒，疫苗等
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