E6783
pFLAG-CMV-3 Expression Vector
shuttle vector for transient or stable expression of N-terminal FLAG
登录查看公司和协议定价
选择尺寸
1 G
¥491.12
5 G
¥1,106.12
25 G
¥3,957.09
100 G
¥13,691.17
¥491.12
目录价¥764.46节省 36%预计发货时间2025年8月15日详情
所有图片(2)
选择尺寸
变更视图
1 G
¥491.12
5 G
¥1,106.12
25 G
¥3,957.09
100 G
¥13,691.17
About This Item
UNSPSC代码:
12352200
¥491.12
目录价¥764.46节省 36%预计发货时间2025年8月15日详情
推荐产品
标签
FLAG® tagged
等级
Molecular Biology
表单
buffered aqueous solution
肽标签位置
N-terminal
运输
dry ice
储存温度
−20°C
1 of 4
此商品 | O7753 | K1750 | O9504 |
|---|---|---|---|
| solubility H2O: 100 mg/mL, clear to slightly hazy, colorless to light yellow | solubility H2O: 100 mg/mL | solubility water: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility H2O: 100 mg/mL |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
一般描述
The pFLAG-CMV™-3 Expression Vector is a 6.3 kb vector used for transient or stable expression in
mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.
The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The FLAG® epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.
Vector Maps and Sequences
mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.
The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The FLAG® epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.
Vector Maps and Sequences
应用
pFLAG-CMV™-3 Expression Vector is suitable for transient or stable expression and secretion of N-terminal FLAG-tagged fusion proteins in mammalian cells.
组分
- pFLAG-CMV™-3 Expression Vector 20 μg (E8770) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CMV™-3-BAP Control Plasmid 20 μg (C3972) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
原理
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG® sequence and directs secretion of the fusion protein into the culture medium. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants.
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
法规信息
新产品
Expression of human MDGA1 increases cell motility and cell-cell adhesion and reduces adhesion to extracellular matrix proteins in MDCK cells.
Diaz-Lopez, A., et al.
Cancer Microenvironment, 4, 23-23 (2011)
Ahmet Sinan Sari et al.
Turkish journal of biology = Turk biyoloji dergisi, 45(3), 301-313 (2021-08-12)
Selective targeting of transfected mesenchymal stem cells (MSCs) carrying specific antioncogenes to the tumor was suggested as a treatment option. Bone morphogenetic protein-2 (BMP2) was shown to inhibit the proliferation and aggressiveness of osteosarcoma (OS) cells. Here, we aimed to
Chenyun Wang et al.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, 32(2), 299-302 (2012-04-25)
Many researchers employed mammalian expression system to artificially express cannabinoid receptors, but immunoblot data that directly prove efficient protein expression can hardly be seen in related research reports. In present study, we demonstrated cannabinoid receptor protein was not able to
Raogo Ouedraogo et al.
Diabetes, 55(6), 1840-1846 (2006-05-30)
Adiponectin is an abundant adipocyte-derived plasma protein with antiatherosclerotic effects. Vascular signal transduction by adiponectin is poorly understood and may involve 5'-AMP-activated protein kinase (AMPK), cAMP signaling, and other pathways. Hyperglycemia sharply increases the production of reactive oxygen species (ROS)
Niu Shin et al.
The Journal of pharmacology and experimental therapeutics, 338(1), 228-239 (2011-04-05)
C-C chemokine receptor 5 (CCR5) is a clinically proven target for inhibition of HIV-1 infection and a potential target for various inflammatory diseases. In this article, we describe 5-[(4-{(3S)-4-[(1R,2R)-2-ethoxy-5-(trifluoromethyl)-2,3-dihydro-1H-inden-1-yl]-3-methylpiperazin-1-yl}-4-methylpiperidin-1-yl)carbonyl]-4,6-dimethylpyrimidine dihydrochloride (INCB9471), a potent and specific inhibitor of human CCR5 that
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持
