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此商品 | C5864 | OGS636 | OGS632 |
|---|---|---|---|
| recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli | recombinant expressed in E. coli |
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in ambient | shipped in ambient |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level - |
一般描述
pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.
The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
Vector Maps and Sequences
The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
Vector Maps and Sequences
组分
- pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
历史批次信息供参考:
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Seung-Gi Jin et al.
The Journal of biological chemistry, 280(13), 12700-12709 (2005-02-11)
MBD2 and MBD3 are two proteins that contain methyl-CpG binding domains and have a transcriptional repression function. Both proteins are components of a large CpG-methylated DNA binding complex named MeCP1, which consists of the nucleosome remodeling and histone deacetylase complex
Lingyan Wang et al.
Communications biology, 4(1), 921-921 (2021-07-31)
Retinoic acid-inducible gene I (RIG-I) senses viral RNA and instigates an innate immune signaling cascade to induce type I interferon expression. Currently, the regulatory mechanisms controlling RIG-I activation remain to be fully elucidated. Here we show that the FAK family
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