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等级
for molecular biology
质量水平
无菌性
0.2 μm filtered
表单
liquid
组成
Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
技术
microbiological culture: suitable
应用
agriculture
储存温度
2-8°C
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
应用
特点和优势
- 经过滤灭菌的即用型培养基
- 便利的包装规格
- 标准配方
相关产品
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Why is SOC Medium (S1797) used to recover bacterial cells before transformation?
SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation. The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
General protocols for growth of competent cells and their transformation (uptake of DNA).
感受态细胞生长及其转化（吸收DNA）的通用实验方案。
实验方案
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
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