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标签
3X FLAG tagged
等级
Molecular Biology
表单
buffered aqueous solution
肽标签位置
N-terminal
运输
dry ice
储存温度
−20°C
一般描述
p3XFLAG-CMV™-10表达载体是pCMV5的一种6.3kb衍生载体，可用于哺乳动物细胞中N-末端Met-3XFLAG™融合蛋白的瞬时或稳定的细胞内表达。该载体可编码多克隆区上游的三个相邻FLAG®表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。这可以增加使用ANTI-FLAG M2抗体的检测灵敏度。第三个FLAG表位上包含肠激酶识别序列，因而可从纯化的融合蛋白中对3XFLAG肽进行切割。当使用SV40 T抗原表达宿主时，复制效率最佳。
p3XFLAG-CMV-7-BAP对照质粒是pCMV5的一种6.2kb的衍生质粒，可用于在哺乳动物细胞中瞬时细胞内表达N末端3X-FLAG细菌碱性磷酸酶融合蛋白。该载体可编码多克隆区上游的三个相邻FLAG表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。这可以增加使用ANTI-FLAG M2抗体的检测灵敏度。第三个FLAG表位上包含肠激酶识别序列，因而可从纯化的融合蛋白中对3XFLAG肽进行切割。
载体图谱和序列
p3XFLAG-CMV-7-BAP对照质粒是pCMV5的一种6.2kb的衍生质粒，可用于在哺乳动物细胞中瞬时细胞内表达N末端3X-FLAG细菌碱性磷酸酶融合蛋白。该载体可编码多克隆区上游的三个相邻FLAG表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。这可以增加使用ANTI-FLAG M2抗体的检测灵敏度。第三个FLAG表位上包含肠激酶识别序列，因而可从纯化的融合蛋白中对3XFLAG肽进行切割。
载体图谱和序列
组分
20 μg p3XFLAG-CMV™-10表达载体 （E4401）以0.5mg/ml的浓度,溶于含有1mM EDTA的10mM Tris-HCl（pH8.0）中的形式提供。
20 μg p3XFLAG-CMV™-7-BAP对照质粒（C7472）以0.5mg/ml的浓度,溶于含有1mM EDTA的10mM Tris-HCl（pH8.0）中的形式提供。
20 μg p3XFLAG-CMV™-7-BAP对照质粒（C7472）以0.5mg/ml的浓度,溶于含有1mM EDTA的10mM Tris-HCl（pH8.0）中的形式提供。
原理
人巨细胞病毒3的启动子调节区可驱动FLAG®-融合构建体的转录。氨基糖苷磷酸转移酶II基因（Neo-r）可表现对氨基糖苷类（如G418）的抗性，从而可用于稳定转染子的选择。
法律信息
该产品涵盖于由Sigma-Aldrich公司所拥有的以下专利中：US6,379,903、US7,094,548、JP4405125、EP1220933、CA2386471和AU774216。
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
法规信息
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