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Merck
CN
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文件

安全信息

F4799

Sigma-Aldrich

3xFLAG™ Peptide

≥90% (HPLC/MS), lyophilized powder

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别名:
Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk肽, dykddddk肽
经验公式（希尔记法）:
C120H169N31O49S
分子量:
2861.87
MDL编号:
MFCD01863911
UNSPSC代码:
12352202
NACRES:
NA.32

product name

3X FLAG® 肽, lyophilized powder

检测方案

≥90% (HPLC/MS)

质量水平

200

形式

lyophilized powder

浓度

(Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.)

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

3X FLAG肽是一种具有23个氨基酸残基的合成肽。在该肽中Asp-Tyr-Lys-Xaa-Xaa-Asp基序重复了三次。在C-末端的八个氨基酸组成了经典的FLAG序列(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)。

应用

用于从在溶液中的或结合至抗-FLAG® M2琼脂糖亲和凝胶（A2220）上琼脂糖上的抗-FLAG ®M2单克隆抗体（F1804）进行3X FLAG®融合蛋白的竞争性洗脱。通过亲和色谱法可实现这一目标。用于将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上温和的洗脱。1X FLAG肽（F3290）将不会把3X FLAG融合蛋白从抗-FLAG®亲和凝胶上洗脱下来。


请访问我们的FLAG® 应用 门户网站，了解更多产品详情。

制备说明

如需制备储备溶液，可溶解于TBS（含有150 mM NaCl的50mM Tris-HCl，pH7.4）至5 mg/mL的浓度。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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Yannick Doyon et al.
Molecular and cellular biology, 24(5), 1884-1896 (2004-02-18)
The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost
Min Hwa Shin et al.
PLoS genetics, 12(2), e1005884-e1005884 (2016-03-02)
The inactivation of p53 creates a major challenge for inducing apoptosis in cancer cells. An attractive strategy is to identify and subsequently target the survival signals in p53 defective cancer cells. Here we uncover a RUNX2-mediated survival signal in p53
Andrew T Schiffmacher et al.
The Journal of cell biology, 215(5), 735-747 (2016-11-20)
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest cells reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, to permit their EMT and migration. Serial processing of Cad6B by a
Oriane Mauger et al.
Nucleic acids research, 43(3), 1869-1882 (2015-01-22)
Alternative splicing is the main source of proteome diversity. Here, we have investigated how alternative splicing affects the function of two human histone methyltransferases (HMTase): G9A and SUV39H2. We show that exon 10 in G9A and exon 3 in SUV39H2
Paul L Colbert et al.
Oncotarget, 6(2), 953-968 (2014-12-02)
Microtubules (MTs) are components of the cytoskeleton made up of polymerized alpha and beta tubulin dimers. MT structure and function must be maintained throughout the cell cycle to ensure proper execution of mitosis and cellular homeostasis. The protein tyrosine phosphatase
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商品

Anti-FLAG® M2磁珠

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

实验方案

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀_蛋白纯化-默克生命科学

免疫沉淀（IP）可用于高效、高产量分离和纯化与FLAG®肽标签融合的蛋白质。此过程采用ANTI-FLAG® M2亲和凝胶进行，后者是一种与琼脂糖树脂共价结合的高度特异性单克隆抗体。

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