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Merck
CN

F1804

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

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别名:
flag抗体, 抗 ddddk, 抗 dykddddk
UNSPSC代码:
12352203
NACRES:
NA.32

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

纯化方式

affinity chromatography (affinity tag purification)

种属反应性

all

浓度

1 mg/mL

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

wet ice

储存温度

−20°C

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一般描述

小鼠抗-FLAG M2抗体,可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。

纯化方法 - 亲和标签纯化

特异性

结合位点:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

免疫原

FLAG;肽序列DYKDDDDK

应用

用于通过免疫印迹、免疫沉淀(IP)、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。 优化用于哺乳动物、植物和细菌表达系统中FLAG融合蛋白的单条带检测。

蛋白质免疫印迹和EIA

更多产品信息请访问我们的FLAG®应用页面。

外形

在磷酸盐缓冲液中含有50%甘油。

制备说明

在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液(TBS),pH 8.0

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Brianna Lowey et al.
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Hepatitis C virus (HCV) harnesses host dependencies to infect human hepatocytes. We previously identified a pivotal role of IκB kinase α (IKK-α) in regulating cellular lipogenesis and HCV assembly. In this study, we defined and characterized NF-κB-inducing kinase (NIK) as
Monika Srivastava et al.
Nature communications, 6, 6253-6253 (2015-02-24)
Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)
Histone H1 Limits DNA Methylation in Neurospora crassa
Michael Seymour
G3 (Bethesda, Md.) (2016)
Yumin Qiu et al.
Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as

商品

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

免疫印迹(蛋白质印迹转印)是现代蛋白质组学研究中的常用技术。

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蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

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