F1804

单克隆抗-FLAG^® M2 小鼠抗

Name: 单克隆抗-FLAG<SUP>®</SUP> M2 小鼠抗
Brand: SIGMA

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

别名:

flag抗体, 抗 ddddk, 抗 dykddddk

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关于此项目

NACRES:

NA.32

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

M2, monoclonal

Application:

—

Citations:

9676

主要文件

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biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

General description

小鼠抗-FLAG M2抗体，可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。

纯化方法 - 亲和标签纯化

Immunogen

FLAG；肽序列DYKDDDDK

Application

用于通过免疫印迹、免疫沉淀（IP)、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。优化用于哺乳动物、植物和细菌表达系统中FLAG融合蛋白的单条带检测。

蛋白质免疫印迹和EIA

更多产品信息请访问我们的FLAG^®应用页面。

Biochem/physiol Actions

结合位点：N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

在磷酸盐缓冲液中含有50%甘油。

Preparation Note

在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液（TBS），pH 8.0

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

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历史批次信息供参考:

分析证书(COA)

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Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.

Monika Srivastava et al.

Nature communications, 6, 6253-6253 (2015-02-24)

Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances

HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway

Yalan Liu

Virology, 154-154 (2016)

ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins

Aparna A

Nucleic Acids Research, 45(7), 4189-4201 (2017)

Histone H1 Limits DNA Methylation in Neurospora crassa

Michael Seymour

G3 (Bethesda, Md.) (2016)

TRIM50 acts as a novel Src suppressor and inhibits ovarian cancer progression.

Yumin Qiu et al.

Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)

Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as

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商品

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

使用抗体 Atto 染料缀合物进行荧光多重检测

免疫印迹（蛋白质印迹转印）是现代蛋白质组学研究中的常用技术。

单克隆抗-FLAG® M2 小鼠抗

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

选择尺寸

关于此项目

主要文件

属性

biological source

conjugate

antibody form

antibody product type

clone

form

purified by

species reactivity

concentration

isotype

immunogen sequence

shipped in

storage temp.

Quality Level

相关类别

说明

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Preparation Note

Other Notes

Legal Information

安全信息

存储类别

wgk

flash_point_f

flash_point_c

法规信息

文件

分析证书(COA)

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单克隆抗-FLAG^® M2 小鼠抗