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F1804

Sigma-Aldrich

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):

flag antibody, Anti-ddddk, Anti-dykddddk

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50 μG
CN¥1,441.96
200 μG
CN¥6,037.78
1 MG
CN¥12,860.28
5 MG
CN¥19,933.74

CN¥1,441.96

Estimated to ship onJune 13, 2025Details

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50 μG
CN¥1,441.96
200 μG
CN¥6,037.78
1 MG
CN¥12,860.28
5 MG
CN¥19,933.74

About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

CN¥1,441.96

Estimated to ship onJune 13, 2025Details

Request a Bulk Order

biological source

mouse

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1 mg/mL

isotype

IgG1

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This Item
F3165F9291A2220
Monoclonal ANTI-FLAG® M2 antibody produced in mouse 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

F1804

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Monoclonal ANTI-FLAG® M2 antibody produced in mouse clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

F3165

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous glycerol solution

F9291

Monoclonal ANTI-FLAG® BioM2 antibody produced in mouse

ANTI-FLAG® M2 Affinity Gel purified immunoglobulin, buffered aqueous glycerol solution

A2220

ANTI-FLAG® M2 Affinity Gel

biological source

mouse

biological source

mouse

biological source

mouse

biological source

-

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

-

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting[1], immunoprecipitation (IP) [2][3][4], immunohistochemisty, immunofluorescence[5] and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG® application portal.

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000427824
    0000427824
    0000427827
    0000427827
    0000425395

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    G3 (Bethesda, Md.) (2016)
    MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBP?
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    ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
    Aparna A
    Nucleic Acids Research, 45(7), 4189-4201 (2017)
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