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标签
3X FLAG tagged
等级
Molecular Biology
表单
buffered aqueous solution
运输
dry ice
储存温度
−20°C
1 of 4
此商品 | 2752M | 11669915001 | 11647229001 |
|---|---|---|---|
| manufacturer/tradename Calbiochem® | manufacturer/tradename Chemicon® | manufacturer/tradename Roche | manufacturer/tradename Roche |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| usage sufficient for 1000 tests, sufficient for 200 tests | usage - | usage sufficient for ≤1,000 tests | usage sufficient for ≤1,000 tests |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| storage temp. −20°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
一般描述
p3XFLAG-CMV™-14 表达载体是分子量6.3 kb 的pCMV51衍生物，用于哺乳动物细胞内 C-末端 3XFLAG™融合蛋白的瞬时或稳定胞内表达。该载体编码多克隆区下游三个相邻的 FLAG®表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。当使用SV40 T抗原表达宿主时，可以实现理想的复制效率和基因组整合。
p3XFLAG-CMV-7-BAP 对照质粒是分子量6.2 kb的pCMV5衍生物，用于N末端3XFLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。该载体编码多克隆区上游三个相邻的 FLAG 表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。第三FLAG 表位包括肠激酶识别序列，可以使3XFLAG肽从纯化的融合蛋白上裂解。
载体图谱和序列
p3XFLAG-CMV-7-BAP 对照质粒是分子量6.2 kb的pCMV5衍生物，用于N末端3XFLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。该载体编码多克隆区上游三个相邻的 FLAG 表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。第三FLAG 表位包括肠激酶识别序列，可以使3XFLAG肽从纯化的融合蛋白上裂解。
载体图谱和序列
组分
- p3XFLAG-CMV™-14 表达载体 20 μg（E4901），浓度为 0.5mg/ml，在含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中提供。
- p3XFLAG-CMV™-7-BAP 对照质粒 20 μg（C7472），浓度为 0.5mg/ml，在含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中提供。
原理
人巨细胞病毒的启动子调节区驱动 FLAG®-融合构建体的转录
其他说明
浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。
法律信息
该产品受 Sigma-Aldrich 公司所拥有的以下专利保护：US6,379,903、US7,094,548、JP4405125、EP1220933、CA2386471 和 AU774216。
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
法规信息
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