推荐产品
标签
3X FLAG tagged
等级
for molecular biology
形式
buffered aqueous solution
运输
dry ice
储存温度
−20°C
一般描述
p3XFLAG-CMV™-14 表达载体是分子量6.3 kb 的pCMV51衍生物，用于哺乳动物细胞内 C-末端 3XFLAG™融合蛋白的瞬时或稳定胞内表达。该载体编码多克隆区下游三个相邻的 FLAG®表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。当使用SV40 T抗原表达宿主时，可以实现理想的复制效率和基因组整合。
p3XFLAG-CMV-7-BAP 对照质粒是分子量6.2 kb的pCMV5衍生物，用于N末端3XFLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。该载体编码多克隆区上游三个相邻的 FLAG 表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。第三FLAG 表位包括肠激酶识别序列，可以使3XFLAG肽从纯化的融合蛋白上裂解。
载体图谱和序列
p3XFLAG-CMV-7-BAP 对照质粒是分子量6.2 kb的pCMV5衍生物，用于N末端3XFLAG细菌碱性磷酸酶融合蛋白在哺乳动物细胞中的瞬时细胞内表达。该载体编码多克隆区上游三个相邻的 FLAG 表位 (Asp-Tyr-Lys-Xaa-Xaa-Asp) 。这在采用ANTI-FLAG M2抗体时会导致检测灵敏度上升。第三FLAG 表位包括肠激酶识别序列，可以使3XFLAG肽从纯化的融合蛋白上裂解。
载体图谱和序列
组分
- p3XFLAG-CMV™-14 表达载体 20 μg（E4901），浓度为 0.5mg/ml，在含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中提供。
- p3XFLAG-CMV™-7-BAP 对照质粒 20 μg（C7472），浓度为 0.5mg/ml，在含有 1mM EDTA 的 10mM Tris-HCl（pH8.0）中提供。
原理
人巨细胞病毒的启动子调节区驱动 FLAG®-融合构建体的转录
其他说明
浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。
法律信息
该产品受 Sigma-Aldrich 公司所拥有的以下专利保护：US6,379,903、US7,094,548、JP4405125、EP1220933、CA2386471 和 AU774216。
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
法规信息
新产品
Viorel Simion et al.
JCI insight, 5(21) (2020-10-07)
Long noncoding RNAs (lncRNAs) play important roles in regulating diverse cellular processes in the vessel wall, including atherosclerosis. RNA-Seq profiling of intimal lesions revealed a lncRNA, VINAS (Vascular INflammation and Atherosclerosis lncRNA Sequence), that is enriched in the aortic intima
Irina Malyukova et al.
Investigative ophthalmology & visual science, 47(1), 206-212 (2005-12-31)
The present study compared properties of wild-type and mutated mouse and human myocilin (Myoc) proteins as a prerequisite for development of a mouse model of glaucoma. cDNA encoding full-length mouse Myoc was cloned into the p3XFLAG-CMV-14 vector. Tyr423His and Ile463Ser
James A Snipes et al.
The Journal of pharmacology and experimental therapeutics, 313(2), 668-676 (2005-01-15)
A splice variant of cyclooxygenase-1 (COX-1), COX-1b (previously termed as COX-3), has been identified in canine tissues as an acetaminophen-sensitive isoform, but the sequence of COX-1b mRNA and the encoded protein are not known in rats. We cloned and sequenced
Yan Qun Chen et al.
The Journal of biological chemistry, 283(15), 10048-10057 (2008-02-02)
AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family that appears to be important in triglyceride biosynthesis in several tissues, but the precise biochemical function of the enzyme is unknown. In the current study, we show that AGPAT6 is
Zhi Sheng et al.
Molecular and cellular biology, 25(21), 9419-9426 (2005-10-18)
Fibroblast growth factor 2 (FGF-2), which is highly expressed in developing tissues and malignant cells, regulates cell growth, differentiation, and migration. Five isoforms (18 to approximately 34 kDa) of FGF-2 are derived from alternative initiation codons of a single mRNA.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门