标签
FLAG® tagged
等级
for molecular biology
形式
buffered aqueous solution
运输
dry ice
储存温度
−20°C
一般描述
The pFLAG-ATS™ Expression Vector is a 5.4 kb E. coli expression vector used for cloning and periplasmic expression of properly inserted open reading frame as an N-terminal FLAG fusion protein. The N-terminal FLAG® fusion protein may be detected using Monoclonal ANTI-FLAG M1, Catalog Number F3040, or Monoclonal ANTI-FLAG M2, Catalog Number F3165, or purified using ANTI-FLAG M1 Agarose Affinity Gel, Catalog Number A4596 or ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.
The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
The pFLAG-ATS-BAP Control Plasmid is a 6.8 kb E. coli plasmid used for efficient and controlled periplasmic expression of N-terminal FLAG-BAP fusion protein.
Vector Maps and Sequences
组分
- pFLAG-ATS™ Expression Vector 10 μg ( E5769) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA
- pFLAG-ATS™-BAP Control Plasmid 1 μg (C9079) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-ATS is a trademark of Sigma-Aldrich Co. LLC
WGK
WGK 1
法规信息
新产品
S Davletova et al.
Journal of experimental botany, 52(355), 215-221 (2001-04-03)
A calmodulin like domain protein kinase (CPK) homologue was identified in alfalfa and termed MsCPK3. The full-length sequence of cDNA encoded a 535 amino acid polypeptide with a molecular weight of 60.2 kDa. The deduced amino acid sequence showed all
J L Gommerman et al.
Blood, 91(6), 1891-1900 (1998-04-16)
Steel factor (SLF), the ligand for the c-Kit receptor, protects hemopoietic progenitors and mast cells from apoptosis. We show here that protection of 32D-Kit cells or mast cells from apoptosis by SLF is abrogated through concurrent inhibition of Ca2+ influx.
D M Stewart et al.
The Journal of clinical investigation, 97(11), 2627-2634 (1996-06-01)
The Wiskott-Aldrich syndrome (WAS) is an X-linked disorder characterized by thrombocytopenia, eczema, disorders in cell-mediated and humoral immunity, and a proclivity to lymphoproliferative disease. The gene responsible encodes a 53-kD proline-rich protein of unknown function (WASP). We produced a FLAG-WASP
P J Brown et al.
FEBS letters, 497(1), 31-38 (2001-05-30)
Micronemes are specialised organelles, found in all apicomplexan parasites, which secrete molecules that are essential for parasite attachment to and invasion of host cells. Regions of several microneme proteins have sequence similarity to the Apple domains (A-domains) of blood coagulation
Birendra Singh et al.
Microbiology (Reading, England), 154(Pt 3), 797-809 (2008-03-04)
We describe an ATP-binding cassette (ABC) transporter in Pseudomonas putida KT2440 that mediates the uptake of glutamate and aspartate. The system (AatJMQP, for acidic amino acid transport) is encoded by an operon involving genes PP1071-PP1068. A deletion mutant with inactivated
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