一般描述
应用
适合制备测序用单细胞悬液。
特点和优势
公式变量
制备说明
法律信息
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
For what purposes can Product A6964, Accutase® solution, be used?
It can be used for detaching cells, analysis of cell surface markers and virus growth assays.
Will Product A6964, Accutase® solution, work on tissue?
This product is not tested for tissue, but it should work on soft tissue.
What is the concentration of Product A6964, Accutase® solution?
This product is typically at a concentration of 400-600 units/mL.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Does Product A6964, Accutase® solution, contain animal components?
Accutase is purified from a marine invertebrate. There are no other animal or bacterial derived proteins present.
What is the difference between Accutase and Accumax?
Accutase is a mixture of non-mammalian proteases. Accumax is accutase that has been augmented with DNase to reduce clumping associated with DNA release from damaged cells.
What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
How can I inactivate Accutase® with fetal bovine serum?
Due to its gentle nature Accutase® cell detachment solution usually does not need to be neutralized following cell passaging. Accutase® is also heat inactivated after 45 minutes at 37°C. If this method of neutralization does not work for your cells, or if you want to neutralize Accutase® in a shorter period of time, the addition of 10% FBS containing media will accomplish this task. Experiments have shown that diluting a sample of Accutase® with 10% with fetal bovine serum achieved an environment similar to that found in cell culture. Measurements of the enzyme activity in this diluted sample showed decreased activity over time, falling below that of the required level for cell detachment (>175 units/mL). After about 6 minutes of serum interaction, the enzyme activity of Accutase® was well below this level, demonstrating effective inhibition by FBS.
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