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Merck
CN

A5533

Sigma-Aldrich

茜素红 S

certified by the Biological Stain Commission, powder

别名:

3,4-二羟基-9,10-二氧代-2-蒽磺酸 钠盐, Sodium alizarinsulfonate, 水溶性茜素红, 茜素 S 钠, 茜素磺酸 钠盐, 茜素磺酸钠, 茜素胭脂红

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About This Item

经验公式(希尔记法):
C14H7NaO7S
CAS号:
分子量:
342.26
颜色索引号:
58005
Beilstein:
4117090
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

product name

茜素红 S, certified by the Biological Stain Commission

等级

certified by the Biological Stain Commission

质量水平

形式

powder

浓度

0.015 g/L in 0.1 M NaOH

技术

titration: suitable

颜色

dark red
yellow-orange to dark orange

溶解性

H2O: 1 mg/mL

ε (extinction coefficient)

592-598 nm at 0.015 g/L in 0.1 M NaOH

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].Oc1c(O)c(cc2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O

InChI

1S/C14H8O7S.Na/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;/h1-5,17-18H,(H,19,20,21);/q;+1/p-1

InChI key

HFVAFDPGUJEFBQ-UHFFFAOYSA-M

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应用

  • 茜素红S在成骨培养中用于钙沉积的检测。
  • 它已用于主动脉外植体钙化的定量分析。
  • 它已可用于成骨细胞培养的矿物质染色。

生化/生理作用

茜素红S用于软骨和骨的染色。它可与钙反应,从而有助于诊断钙沉积。它也用于研究碳水化合物-硼酸的相互作用。

适用性

已通过Dahl′s钙测定法认证

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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分析证书(COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Product A5533, Alizarin Red S, is commonly used to stain calcium in tissue sections.  Will other cations such as magnesium, manganese, barium and strontium also be detected?

    No, typically only calcium is present in sufficient quantities to be demonstrated in tissue sections.

  6. Product A5533, Alizarin Red S, has been prepared according to protocol. The solution has gelled or thickened. Is the solution still usable?

    Yes. Although the solution has thickened or gelled, it is still suitable for use.

  7. I see that Product A5533, Alizarin Red S, was tested according to the Dahl method at pH 7.0. Can the dye also be used in the McGee-Russell procedure at pH 4.2?

    Yes. The dye can be used at a variety of pH values.

  8. What are the pH transition ranges for Product A5533, Alizarin Red S?

    Although the pH transition ranges are not determined on a lot to lot basis, the solution changes from yellow to red in the range of  3.5-6.5, and from the orange to violet at pH 9.4-12. On a lot to lot basis there can be considerable difference in regards to color and pH ranges.

  9. When using Product A5533, Alizarin Red S, will amorphous calcium deposits be detected in tissue sections?

    Amorphous calcium phosphate deposits are very soluble below pH 8.5.  In most instances the deposits are lost during either standard fixation or the during the standard staining procedure.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

RGD peptides immobilized on a mechanically deformable surface promote osteoblast differentiation.
Cavalcanti-Adam EA, et al.
Journal of Bone and Mineral Research, 17, 2130-2130 (2002)
Pathologic calcification of adult vascular smooth muscle cells differs on their crest or mesodermal embryonic origin.
Leroux-Berger M, et al.
Journal of Bone and Mineral Research, 26, 1543-1543 (2011)
On the history and mechanism of alizarin and alizarin red S stains for calcium.
Puchtler H, et al.
The Journal of Histochemistry and Cytochemistry, 17, 110-124 (1969)
Impaired marrow osteogenesis is associated with reduced endocortical bone formation but does not impair periosteal bone formation in long bones of SAMP6 mice.
Silva MJ, et al.
Journal of Bone and Mineral Research, 20, 419-419 (2005)
Phenol red inhibits chondrogenic differentiation and affects osteogenic differentiation of human mesenchymal stem cells in vitro.
Lysdahl H, et al.
Stem Cell Reviews, 9, 132-132 (2013)

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