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Merck
CN

SCR005

蛋白酶细胞分离液

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

别名:

Cell detachment solution

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UNSPSC Code:
12352204
NACRES:
NA.78
eCl@ss:
32160801

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产品名称

蛋白酶细胞分离液, A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

form

liquid

manufacturer/tradename

Chemicon®

input

sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells

shipped in

dry ice

Quality Level

300

相关类别

Application

细胞分离

1.将ACCUTASE®在室温下解冻。

2.用无菌PBS洗涤孔板、烧瓶或珠子。

3.通过无菌程序添加ACCUTASE至培养皿或烧瓶中（每75平方厘米表面积10 ml）。

4.经培养物放回37°C培养箱中，以分离细胞（5-10分钟）。

5.按常规操作进行细胞计数和传代。无需额外的洗涤或酶抑制剂



每种细胞/组织类型的最佳孵育时间和试剂强度应有最终用户进行确定。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

含蛋白水解和胶原水解酶的细胞分离溶液。可用于将常规细胞从标准组织培养塑料和粘附涂层塑料（包括SmartPlastic）上进行常规分离。 ACCUTASE 不含有哺乳动物或细菌来源产品。

由于该试剂不包含通用蛋白水解酶，因此可能无法均匀地解离所有细胞/组织类型。通常需要对细胞类型和传代次数以及孵育时间和试剂强度进行一些优化，以优化试剂的有效性。

ACCUTASE对多种细胞类型有效，包括：成纤维细胞、角质形成细胞、血管内皮细胞、肝细胞、血管平滑肌细胞、肝细胞祖细胞、原代鸡胚神经元细胞、骨髓干细胞、贴壁CHO和BHK细胞、巨噬细胞、293细胞、L929细胞、永生小鼠睾丸生殖细胞、3T3、Vero、COS、HeLa、NT2、MG63、M24和A375转移性黑色素瘤、神经胶质瘤U251和D54、HT1080纤维肉瘤细胞以及Sf9昆虫细胞。

每批次的ACCUTASE都已经过无菌性（通过USP膜过滤方法）、酶活性（通过合成发色四肽测试）和细胞与组织培养塑料的分离性能测试。

Physical form

100 ml，即用型，冷冻无菌液体。 1X ACCUTASE酶，溶于含0.5 mM EDTA·4Na和3 mg/L苯酚红的Dulbecco′s PBS（0.2 g/L KCl、0.2 g/L KH2PO4、8 g/L NaCl和1.15 g/L Na2HPO4）中。

Preparation Note

保存于-20°C可保持稳定。请参阅批次到期日期。建议收货时保存于-20°C。解冻后，ACCUTASE可以在4°C下最多保存2个月。请勿在室温下存放

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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