无菌性
sterile-filtered
质量水平
形式
liquid
储存条件
dry at room temperature
浓度
0.4%
技术
cell culture | mammalian: suitable
tissue processing: suitable
应用
cell analysis
运输
ambient
SMILES字符串
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
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相关类别
一般描述
应用
- 细胞活力检测，计数活细胞和死细胞
- 在rescue实验中计数活细胞
- 台盼蓝拒染计数法，测定增殖曲线
- 配制生理盐水对照和脂多糖(LPS)溶液，确认LPS成功输注
- 台盼蓝拒染实验，测定胃上皮细胞(GEC)活力
- 通过血球计数板计数GEC细胞
制备说明
可比产品
警示用语：
Danger
危险声明
危险分类
Carc. 1B
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
Why is Trypan blue, Product T8154, used to determine cell viability?
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
Trypan blue, Product T8154, contains a precipitate. Is it still good to use?
The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why are there red fibers in Product T8154, Trypan blue solution?
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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