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D8537

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

别名:

DPBS

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100 ML
¥157.22
500 ML
¥305.18
1 L
¥562.97
6 X 500 ML
¥1,710.87
6 X 1 L
¥3,248.33
24 X 500 ML
¥6,265.47

¥157.22

预计发货时间2025年4月07日详情

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100 ML
¥157.22
500 ML
¥305.18
1 L
¥562.97
6 X 500 ML
¥1,710.87
6 X 1 L
¥3,248.33
24 X 500 ML
¥6,265.47

About This Item

MDL编号:
MFCD00131855
UNSPSC代码:
12352207
eCl@ss:
32129211
NACRES:
NA.75

¥157.22

预计发货时间2025年4月07日详情

获取大包装报价

产品名称

杜氏磷酸盐缓冲盐水, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

质量水平

300

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

glucose: no (Dextro)
sodium pyruvate: no

运输

ambient

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此商品
D1408D1283D8662
杜氏磷酸盐缓冲盐水 Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

D8537

杜氏磷酸盐缓冲盐水

Sigma-Aldrich

D1408

杜氏磷酸盐缓冲盐水

Sigma-Aldrich

D1283

杜氏磷酸盐缓冲盐水

Sigma-Aldrich

D8662

杜氏磷酸盐缓冲盐水

Quality Level

300

Quality Level

400

Quality Level

400

Quality Level

400

form

liquid

form

liquid

form

liquid

form

liquid

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

d-PBS, Dulbeccos PBS, Dulbeccos Buffer, Phosphate Buffered Saline, buffered saline Dulbeccos, balanced salt solution (BSS), bss for mammalian cells,

DPBS, Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

DPBS, Isotonic saline, cell washing solution, cell suspension solution

一般描述

杜氏磷酸盐缓冲盐水（D-PBS）是一种用于处理和培养哺乳动物细胞的平衡盐溶液（BSS）。DPBS缓冲液可用于冲洗、洗涤并稀释哺乳动物细胞，同时维持渗透平衡。它提供正常细胞代谢所必需的水和无机离子。DPBS的钙和镁有助于细胞的结合和成团。不含这些离子的DPBS可用于悬浮后细胞的洗涤和润洗。磷酸缓冲体系将pH维持在生理性范围内。

应用

Dulbecco′s磷酸盐缓冲液已用于：

  • 稀释新鲜红细胞用于研究红细胞聚集物的捕获。[1]
  • 清洗微量滴定板的微孔[2]
  • 在裂解缓冲液中重悬细胞以前清洗细胞进行DNA分离[3]
  • 作为胚胎保存培养基的成分用于胚胎的玻璃化。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

通常也和此产品一起购买

产品编号
说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    0000408898
    0000408898
    0000365401
    0000365401
    0000401409

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. What is the formulation for Product D8537, Dulbecco's Phosphate?

      The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.    

    4. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

      This is a 1X concentration. The 10X concentration is listed under product D1408.

    5. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

      The pH will be in the range of 7.1-7.5, according to the specification sheet.

    6. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    7. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    8. What is a Salt Toxicity Test?

      The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted.  They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour.  The cells are counted and %viability determined.  The cells are then cultured for an additional 7 hours (total of 8 hours).  The cells are counted again and the % viability determined.  The viability at 1 hr has to be >95% of the original viability.  The viability at 8 hrs must be >75% of the original viability.

    9. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

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    Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
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    Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
    The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
    Saabah B Mahbub et al.
    Scientific reports, 7(1), 15792-15792 (2017-11-19)
    Bioimaging using endogenous cell fluorescence, without any external biomarkers makes it possible to explore cells and tissues in their original native state, also in vivo. In order to be informative, this label-free method requires careful multispectral or hyperspectral recording of
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    人毛囊真皮乳头细胞解冻、传代培养和冻存的技术信息。

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