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D5652

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

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别名:
DPBS
MDL编号:
MFCD00131855
UNSPSC代码:
12352207
eCl@ss:
32129211
NACRES:
NA.25

质量水平

500

形式

powder

技术

cell culture | mammalian: suitable

pH值（酸碱度）

7.2-7.8

溶解性

water: 9.6 g/L, clear

储存温度

2-8°C

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相关类别

一般描述

杜氏(Dulbecco′s)磷酸盐缓冲液(DPBS)是在组织培养中广泛使用的平衡盐溶液(BSS)。

应用

在制备和连续转移过程中，可使用D-PBS清洗细胞。
杜氏磷酸盐缓冲液已用于：
  • 作为模拟生理溶液成分,体外研究Fe–20Mn–1.2C合金的降解行为
  • 溶解朝鲜当归(Angelica gigas)粉末进行微囊化
  • 在细胞培养物处理、愈伤试验、transwell试验和凋亡试验中用于清洗细胞
  • 洗涤羊膜用于细胞分析

生化/生理作用

杜氏磷酸盐缓冲液可维持细胞渗透压平衡，作为灌洗、运输和稀释用液。它提供正常细胞代谢必需的水和大量无机离子。混入葡萄糖等糖后，这种缓冲盐可提供细胞代谢所需的主要能量。它的缓冲能力可使培养基维持在生理pH范围内。

数量

按照每升培养基9.6克粉末配制。

其他说明

该D-PBS配方中没有Ca或Mg。也可提供加入Ca和Mg的配方

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?

    Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.

  6. Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?

    Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use.  It can be autoclaved at 121°C, 15 psi for 20 minutes.

  7. Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?

    Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.

  8. What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?

    This product contains 8.0 g/L sodium chloride.

  9. What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?

    The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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