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P4417

Sigma-Aldrich

Phosphate buffered saline

tablet, pH 7.2-7.6 (1 tablet/200 mL)

别名:

PBS

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About This Item

MDL编号:
MFCD00131855
UNSPSC代码:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, tablet

形式

tablet

质量水平

200

储存条件

dry at room temperature

颜色

white

pH值（酸碱度）

7.2-7.6 (1 tablet/200 mL)

溶解性

water: soluble

密度

1.02 g/cm3 at 20—25 °C
1.03 g/cm3 at 20—25 °C

应用

diagnostic assay manufacturing
histology
life science and biopharma

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相关类别

一般描述

磷酸盐缓冲液（PBS）是一种在生物学研究中得到广泛应用的缓冲溶液，其多功能性和关键属性得到高度认可。特别值得一提的是，其等渗性与人体的pH、渗透压和离子浓度密切相关，它对细胞没有毒性。这种关键特性在多种细胞生物学实验中起到关键作用，包括细胞的温和清洗、组织的安全转运以及细胞和试剂的精确稀释。这些应用有助于保持细胞活力和构建有利的生理环境。PBS的缓冲能力来自于磷酸盐-磷酸的平衡，为实现最佳酶活性和各种生物学过程创建稳定的pH 环境。这种特性令PBS成为试剂制备、蛋白质透析和免疫组织化学等研究工作的理想选择,确保了实验结果的再现性和可靠性.

除了细胞生物学领域外，PBS的多功能性还体现在各种应用中，如为实现最佳抗体-抗原相互作用而构建定制化ELISA缓冲液。此外，它可用作蛋白A/G色谱的高效缓冲液，有助于基于特定抗体结合纯化抗体。在血液学和生物化学操作过程中，PBS 在细胞和组织的有效清洗中起关键作用。总之，PBS是生物学和生物化学研究中不可缺少的工具，具有等渗性、缓冲能力、简单和稳定的特性。其在细胞生物学、试剂制备、蛋白质纯化和各种生物分析中的多样化应用体现了其在增进科学知识和理解中的重要性。

应用

磷酸缓冲盐已用于-
  • 生产铂纳米颗粒(NP-Pt)水解胶体
  • 在SEM之前清洗血液样品和SEM（扫描电子显微镜）存根
  • 制备荧光标记小麦胚芽凝集素(WGA)溶液
  • 在ELISA（酶联免疫吸附测定）期间稀释捕获抗体
  • 在离心过程中悬浮细胞沉淀
  • 免疫测定程序

特点和优势

  • 适用于各种研究应用的优质产品
  • 可用作生物学和生物化学应用的缓冲液成分
  • 易溶于水

重悬

将一片溶于200ml去离子水中，在25 °C下得到0.01m磷酸盐缓冲液、0.0027m氯化钾和0.137m氯化钠，pH值为7.4。

其他说明

如需了解生化试剂系列的更多信息，请填写此表。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P4417, Phosphate buffered saline (PBS), contain calcium or magnesium?

    No.  The complete formulation is given in the product listing.  No calcium or magnesium salts are included in the preparation.

  4. Does one tablet of Product P4417, Phosphate buffered saline (PBS), make up 1X or 10X PBS?

    When 1 tablet is reconstituted in 200 ml of water, the resulting solution is 1X PBS.

  5. Can Product P4417, Phosphate buffered saline (PBS), solution be autoclaved?

    Yes.  PBS is not harmed by standard autoclave procedures.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

M Michaelis et al.
The journal of physical chemistry. B, 123(31), 6694-6704 (2019-07-11)
Although successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over
Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.
Kutwin M et al
Nanoscale Research Letters, 9(1), 257-257 (2014)
Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.
Lopez C et al
Food Chemistry, 120.1, 22-33 (2010)
Simon C Drew et al.
PloS one, 5(12), e15875-e15875 (2011-01-07)
The amyloid-β (Aβ) peptide is the primary component of the extracellular senile plaques characteristic of Alzheimer's disease (AD). The metals hypothesis implicates redox-active copper ions in the pathogenesis of AD and the Cu(2+) coordination of various Aβ peptides has been
Wenwen Yu et al.
Food chemistry, 241, 493-501 (2017-09-30)
The conversion of barley starch to sugars is a complex enzymic process. Most previous work concerned the biotechnical aspect of in situ barley enzymes. However, the interactions among the macromolecular substrates and their effects on enzymic catalysis has been little
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