生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, rat
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable
western blot: suitable
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... BDNF(627)
特异性
Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 1% cross-reactivity against NGF, NT3 or NT4.
SPECIES REACTIVITIES: Rat and human. Other species not yet tested. It is likely that the antibody will also work on mouse and porcine due to sequence homology.
SPECIES REACTIVITIES: Rat and human. Other species not yet tested. It is likely that the antibody will also work on mouse and porcine due to sequence homology.
免疫原
Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
应用
Detect Brain Derived Neurotrophic Factor using this Anti-Brain Derived Neurotrophic Factor Antibody validated for use in WB, IH.
Immunohistochemistry: 1:1,000 (see suggested protocol).
Immunoblotting:1:1,000; we recommend preparing the BDNF samples for western blot by the method of Katoh-Semba (1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C.
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Immunoblotting:1:1,000; we recommend preparing the BDNF samples for western blot by the method of Katoh-Semba (1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C.
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
外形
Lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water.
储存及稳定性
Maintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
Escalated or suppressed cocaine reward, tegmental BDNF, and accumbal dopamine caused by episodic versus continuous social stress in rats.
Miczek, KA; Nikulina, EM; Shimamoto, A; Covington, HE
The Journal of Neuroscience null
Jessica Mary Livingston-Thomas et al.
Acta pharmacologica Sinica, 34(1), 104-112 (2012-10-30)
Constraint-induced movement therapy (CIMT), which forces use of the impaired arm following unilateral stroke, promotes functional recovery in the clinic but animal models of CIMT have yielded mixed results. The aim of this study is to develop a refined endothelin-1
Fenghua Chen et al.
The international journal of neuropsychopharmacology, 23(7), 446-458 (2020-03-28)
Electroconvulsive therapy (ECT) is a highly effective and fast-acting treatment for depression used in the clinic. Its mechanism of therapeutic action remains uncertain. Previous studies have focused on documenting neuroplasticity in the early phase following electroconvulsive seizures (ECS), an animal
Tom Verhovshek et al.
Developmental neurobiology, 73(8), 587-598 (2013-03-21)
We have previously demonstrated that brain-derived neurotrophic factor (BDNF) interacts with testosterone to regulate dendritic morphology of motoneurons in the highly androgen-sensitive spinal nucleus of the bulbocavernosus (SNB). Additionally, in adult male rats testosterone regulates BDNF in SNB motoneurons and
Ryan G Soderquist et al.
Journal of biomedical materials research. Part A, 91(3), 719-729 (2008-12-03)
Brain-derived neurotrophic factor (BDNF) was covalently attached to polyethylene glycol (PEG) in order to enhance delivery to the spinal cord via the cerebrospinal fluid (intrathecal administration). By varying reaction conditions, mixtures of BDNF covalently attached to one (primary), two (secondary)
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