推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, rat
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable
western blot: suitable
UniProt登记号
1 of 4
此商品 | PHR1383 | A8626 | A2786 |
---|---|---|---|
technique(s) cell culture | plant: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) UV/Vis spectroscopy: suitable | technique(s) cell culture | mammalian: suitable |
assay ≥99% | assay - | assay ≥99% | assay ≥99% |
form powder | form - | form powder | form powder |
application(s) agriculture | application(s) pharmaceutical (small molecule) | application(s) - | application(s) - |
solubility 0.5 M HCl: soluble 20 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility - | solubility 0.5 M HCl: soluble 20 mg/mL, Grade III, colorless to faint yellow or tan | solubility 0.5 M HCl: soluble 20 mg/mL, clear to slightly hazy, colorless to faintly yellow |
mp >360 °C (lit.) | mp >360 °C (lit.) | mp >360 °C (lit.) | mp >360 °C (lit.) |
特异性
SPECIES REACTIVITIES: Rat and human. Other species not yet tested. It is likely that the antibody will also work on mouse and porcine due to sequence homology.
免疫原
应用
Immunoblotting:1:1,000; we recommend preparing the BDNF samples for western blot by the method of Katoh-Semba (1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C.
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Neuroscience
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
外形
储存及稳定性
法律信息
免责声明
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储存分类代码
10 - Combustible liquids
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